反相高效液相色谱法测定枸杞中有机酸的含量.pdf

, Journal ofAnhuiAgr.i Sci. 2010, 38( 1 ): 5 - 60 马树梅 况玲玲 * 董秀丽, 陈向明 (, 264003) [目的]建立反相高效液相色谱法测定有机酸含量的方法[方法]采用 ultmi ate-C 色谱柱, 以磷酸 缓冲溶液( pH= 2. 0)为流动 18 相, 流速 0. 5 m l/m in, 检测波长 210 nm, 建立草酸酒石酸等 7种有机酸的高效液相色谱分析方法, 并对枸杞中几种有机酸进行了分析 [结果]在 22m in内实现了 7种有机酸的基线分离, 检测限为 0. 001~ 0. 03 mg/L[结论]该方法具有分析速度快, 线性范围宽, 重现性 好灵敏度高的优点, 适用于枸杞中有机酸含量的测定分析 高效液相色谱; 有机酸; 枸杞 S567. 17 A 0517- 6611( 2010) 1 - 0 5 - 02 Analysis ofOrganic Acids inM edlar byReversed-Phase High Performance Liquid Chromatography DO G Xiu-li et a l ( B inzhouM edicalUn iversity, Y anta,i Shandong 264003) Abstract [ Objective] The ami was to estab lish amethod of organic acids determ ination by reversed-phase high perform ance liquid chromatog- raphy. [M ethod] A u ltmi ate-C18 column was used w ith phosphate buffer solution ( pH = 2. 0) as themobile phase at a flow rate of 0. 5m l/m in and detection wavelength of 210 nm to establish m ethod for the determ ination of seven organic acids, and organic acids inM edlarw ere ana- lyzed. [ Result] The seven organic acidsw as determ ined in 22 m in and the detection lmi itswere between 0. 001- 0. 03 mg/L. [ Conclusion] Themethod is fas,t sensitive, reproducib le and practical for the routine analysis of organ ic acids in m edlar. Keywords H igh performance liqu id chromatography; Organic acids; M edlar , ,, , , , , [ 1] [ 2] [ 3] [ 4- 6] 1. 3. 3 , , ; 6( 20 Ll), ,; , RSD , ; 1. 3. 4 1g, , 20m l, 40 m in, 25m l20 , Ll,0. 45 Lm , , 1. 3. 5 6, / 1. 3. 10 , ,,