基于Miseq法纳豆冻干粉对小鼠肠道菌群影响的研究黄占旺王素贞.DOC

基于Miseq法纳豆冻干粉对小鼠肠道菌群影响的研究文章篇幅较长，建议精简 文章篇幅较长，建议精简 黄占旺，王素贞，吴高峰，吴少福，黄永平，陈佳妮 （江西农业大学食品科学与工程学院；江西省天然产物与功能食品重点实验室，南昌 330045) 摘要：抗生素近年来被滥用，它抑制或杀死微生物使得机体菌群失调。因此找出一种抗生素的安全有效替代物非常有必要，而益生菌是一种有效的替代物。本研究以纳豆冻干粉为原料，正常小鼠和肠道菌群失衡小鼠为动物试验模型，采用体内灌胃试验和Miseq方法研究纳豆冻干粉对小鼠肠道菌群的影响并进行生物信息分析。分析多样本间的肠道菌群多样性和单样本属水平上的分类情况；分析纳豆芽孢杆菌及益生菌、致病菌的菌数变化情况。预期的研究结果为揭示纳豆芽孢杆菌的肠道菌群调节机理提供科学依据，对人类健康和传统发酵食品微生物的开发具有十分重要的意义。 关键字：纳豆冻干粉 肠道菌群 Miseq法 多样性 中图分类号：R151 文献标识码：A 文章编号：1003-0174 The study of the regulation of intestinal flora in mice by Natto Lyophilized Powder basing on the miseq method基金项目 基金项目 国家自然科学基金 通讯作者：黄占旺，男，1964年出生，教授，食品微生物与发酵代谢产物 Huang Zhanwang, Wang Suzhen, Wu Gaofeng, Wu Shaofu, Huang Yongping, Chen Jiani (Jiangxi Key Laboratory of Natural Products and Functional Food, College of Food Science and Engineering, Jiangxi Agricultural University，Nanchang 330045) Abstract: Antibiotics abuse in recent years, it inhibit or kill microbes that made the body dysbacteriosis. So looking for a safe and effective antibiotic replacement is very necessary,and probiotics is an effective alternative. Natto lyophilized powder is the raw material and the normal mice and low intestinal flora in mice being used as animal testing model, the regulation of intestinal flora in mice by natto lyophilized powder and miseq method were studied using in vivo experimental. Further the biological information of mice was analyzed. The diversity of intestinal flora among multiple samples and the classification of the single samples level were analyzed. The situation of the number of bacteria which was changed in Bacillus natto and probiotics and pathogens were analyzed. Ultimately, the situation of the number of bacteria which was changed in Bacillus natto and probiotics and pathogens was analyzed. Reveal the expected the results of the study on the intestinal flora regulation mechanism of bacillus provided the scientific basis, has a very vital significance for human health and the development of the traditio