苦参碱联合X线对肝癌细胞协同杀伤作用的-肿瘤防治研究.PDF

442 2015 42 5  Cancer Res Prev Treat,2015,Vol.42,No.5 · · 肿瘤防治研究 年第 卷第 期 doi:10.3971/j.issn.1000-8578.2015.05.004 ·基础研究· X 苦参碱联合 线对肝癌细胞协同杀伤作用的 实验 1 2 2 3 2 汪丽菁，程向东 ，徐志远 ，应莉莎 ，谢华夏 Synergistic Killing Effect and Mechanism of Matrine Combined with X-ray on HepG2 Cells WANG Lijing1 2 2 3 2 , CHENG Xiangdong , XU Zhiyuan , YING Lisha , XIE Huaxia 1. Department of Ultrasound, Zhejiang Cancer Hospital, Hangzhou 310022, China; 2. Department of Gastrointestinal Surgery, The First Affiliated Hospital of Zhejiang Chinese Medicine University, Hangzhou 310006, China; 3. Zhejiang Cancer Research Institute, Zhejiang Cancer Center, Hangzhou 310022, China Corresponding Author: CHENG Xiangdong, E-mail: chengxd516@126.com Abstract: Objective To investigate the synergistic killing effect and mechanism of matrine combined with X-ray on human hepatic cancer cells HepG2. Methods MTT and ELISA were used to detect cell viability and apoptosis, respectively. Real-time RT-PCR was used to examine Bcl-2 and Bax expression. Western blot was used to determine the expression of caspase-9, pro-caspase-9 and cytochondrial-C. NobiFlow GOT - IFCC and NobiFlow LDH - L were used to measure the levels of LDH and AST. Results Pretreatment of matrine (3 mg/ml) for 12h significantly increased the killing effect of X-ray on HepG2 cells, induced cells apoptosis, inhibited the expression of Bcl-2, and enhanced the levels of cytochondrial-C and caspapase-9 in the cytoplasm. However, the combination of matrine and X-ray did not alter cell morphology, LDH, or AST expression in human hepatocytes. Conclusion The combination treatment of matrine and X-ray could synergistically inhibit the growth of HepG2 cells by inducing