实用生物医学实验技术-Virus production and transduction.ppt

Lentivirus?(lente-, Latin for slow) is a?member?of Retrovirus?family, characterized by a long?incubation period. The?virions?are spherical and 80–100?nm in diameter, enveloped, with tiny?spikes?(about 8?nm) Lentiviruses can deliver a significant amount of?viral?RNA into the?DNA?of the?host cell?and being able to infect non-dividing cells. Lentivirus expression system is a useful research tool to introduce a gene product or shRNA into?cells or animal models for prolonged expression 慢病毒 (Lentivirus) 慢病毒结构 Genome ss-RNA, three main genes coding for the viral proteins in the order: 5′-gag-pol-