蒈烯对铜绿假单胞菌的抑菌活性及机理初探热带作物学报.pdf

热带作物学报 2019, 40(3): 601608 Chinese Journal of Tropical Crops 3-蒈烯对铜绿假单胞菌的抑菌活性及机理初探 1 1 3 1,2 1,2 1,2 1,2* 刘 雪 ，何英兰 ，胡月英 ，陈卫军 ，陈海明 ，钟秋平 ，陈文学 1. 海南大学食品学院，海南海口 570228 ；2. 海南省春光农产品加工研究院，海南文昌 571333 ；3. 海南大学材料与化工 学院，海南海口 570228 摘 要 为了开发一种新的食品防腐保鲜剂，本文初步研究了 3-蒈烯对铜绿假单胞菌的抑菌活性及机理。通过肉汤稀 释法测定最小抑菌浓度（MIC ）和最小杀菌浓度（MBC ），评价了3-蒈烯的抑菌活性，考察了3-蒈烯对细菌生长曲线、 细胞形态、细胞膜通透性、膜电位及呼吸链脱氢酶等的影响。结果表明：3-蒈烯对铜绿假单胞菌的MIC 为20 mL/L ， MBC 为40 mL/L 。3-蒈烯可以抑制铜绿假单胞菌的生长，破坏细胞的正常形态，提高细胞膜的通透性，造成蛋白质、 电解质的外泄。3-蒈烯能够降低铜绿假单胞菌菌体的膜电位，干扰细胞正常代谢活动，还能抑制细胞内呼吸链脱氢酶 的活性。综上所述，3-蒈烯通过破坏铜绿假单胞菌细胞膜，抑制细菌正常生长，导致细菌细胞死亡。 关键词 3-蒈烯；铜绿假单胞菌；抑菌活性；抑菌机理；细胞膜 中图分类号 TS205.9 文献标识码 A Primary Antibacterial Activity and Mechanism of 3-carene Against the Pseudomonas aeruginosa 1 1 3 1,2 1,2 1,2 LIU Xue , HE Yinglan , HU Yueying , CHEN Weijun , CHEN Haiming , ZHONG Qiuping , CHEN Wenxue1,2* 1. College of Food Science and Technology, Hainan University, Haikou, Hainan 570228, China; 2. Chunguang Agro-Product Proc- essing Institute, Wenchang, Hainan 571333, China; 3. College of Materials and Chemical Engineering, Hainan University, Haikou, Hainan 570228, China Abstract In order to develop natural food preservatives, the primary antibacterial activity and mechanism of 3-carene against Pseudomonas aeruginosa were studied. The antibacterial activity of 3-carene was evaluated based on the mini- mum inhibitory concentration (MIC) and minimum bactericide concentration (MBC) by broth dilution method. The ef- fect of 3-carene was investigated by bacterial growth curve, scanning electron microscopy, electric conductivity, mem- brane potential and respiratory chain dehydrogenase. The results showed that: the MIC and MBC of 3-carene against Pseudomonas aeruginosa were 2