蒲圻贝母组织培养鳞茎形成过程中生理生化变化研究-植物科学学报.pdfVIP

1999, 17(4) : 314318 J ournal of Wuhan Botanical Resear ch 蔡朝晖　李　萍　李会军　高山林 董婷霞　詹华强 ( 210038) ( ) , , PAL , , , , B ulbus f ritillar iae , , Fr itillar ia p uqiensis , , , , , , , , 3 mg/ kg, 1 h , 4 , 3060 / , h mg kg 1, 2 , , , , , , 1 1. 1 Fr itillar ia p uq iensis , 1.2 ( 1) : 3 h,0. 1% 20 min , 1 1 5 ; 0. 5 cm0. 5 cm0.2 cm , MS BA 2 mg L , IAA 1mg L , 25 , 50 d 1 , , ( 2) : MS, 100 mL (40 mL / ) , 50 d 3 , : 1998 10 12, : 1998 12 14: , 1964 , ( ) , 4 : 315 ( 3) : 80% , , , 15 min, 4, 5 , 2. 5 mL, 6.5 mL, 1015 m in, 640 nm , NaOH , 5 min , = / 0. 95 ( 4) 6 ( 5) (PAL) 7 ( 6) 8 2 2. 1 2 , 22 d 45 , ( 1) 45 d , 3035 d 2 A. 22 d ( Bulbs formed at the 22th day) ; B. 50 d ( Bulbs after 50 days) 1 . MS F p uqiensis Fig . 1Bulbs of F .p u qiensis cultured on MS medium ( Raising of gr owth rate) ; ( Growth rate) 2 Fig. 2The changes of growth rate at different culture time 316 17 2.2 1 , , , BA IAA