赤芍总苷退黄降酶的作用及机制研究-中国现代应用药学.pdf

赤芍总苷退黄降酶的作用及机制研究 罗琳 1 ，窦志华 2 ，吴锋 1 ，刘峥 1 ，张伟 1 (1.南通大学，江苏 南通 226001 ；2.南通大学附属南通第三医院，江苏 南通 226001) 摘要：目的 探讨赤芍总苷退黄降酶的作用及机制。方法 ICR 小鼠随机分成正常对照组，模型对照组，赤芍总苷低、中、 高剂量组和苯巴比妥钠组；灌胃 α-萘异硫氰酸酯(ANIT)诱导小鼠黄疸模型，分别在实验第 6 天和第 12 天观察各组药物对小 鼠总胆红素(TBIL)、直接胆红素(DBIL)、胆汁酸(TBA)、丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、碱性磷酸酶(ALP)等 血清学指标及对小鼠胆汁分泌量和肝组织中尿苷二磷酸葡萄糖酸转移酶(UDPG-T)活性的影响，实验第 6 天观察各组药物对 戊巴比妥钠睡眠时间的影响。结果 与模型对照组比较，除第 6 天赤芍总苷中剂量组 ALT 、第 12 天低剂量组 AST 无显著 差异外，赤芍总苷 3 个剂量组血清学指标均明显下降(P0.05 或 P0.01) ；赤芍总苷中、高剂量组胆汁分泌量显著增加(P0.01 或 P0.05) ；3 个剂量组和苯巴比妥钠组睡眠时间均明显缩短(P0.01) ；赤芍总苷组肝组织 UDPG-T 活性明显提高(P0.05) 。 结论 赤芍总苷具有退黄降酶作用，其作用机制可能与增加胆汁分泌量、提高肝药酶及 UDPG-T 活性有关。 关键词：赤芍总苷；退黄降酶；胆汁分泌量；睡眠时间；尿苷二磷酸葡萄糖酸转移酶 中图分类号：R285. 5 文献标志码：A 文章编号：1007-7693(2010)04-0285-04 Study on the Jaundice-Relieving and Enzyme-Reducing Effect and Its Mechanism of Total Paeony Glycoside 1 2 1 1 1 LUO Lin , DOU Zhihua , WU Feng , LIU Zheng , ZHANG Wei (1.Nantong University, Nantong 226001, China; 2.Nantong Third Affiliated Hospital of Nantong University, Nantong 226006, China) ABSTRACT: OBJECTIVE To research the jaundice-relieving and enzyme-reducing effect and its mechanism of total paeony glycoside (TPG). METHODS All ICR mice were randomly divided into normal group, model group, low dose group of TPG (LG), middle dose group of TPG (MG), high dose group of TPG(HG), and phenobarbital sodium group. Intrahepatic cholestatic model of mice was established after ig alpha-naphthylisothiocyanate (ANIT). Effect of drugs on TBIL, DBIL, TBA, ALT, AST, and ALP in serum, capacity of bile secretion, and uridine diphosphoglucuronyl transferase (UDPG-T) activity of liver tissue were observed on the sixth and twelfth day, respectively. Sleeping time of pentobarbitol sodium were observed on the sixth day. RESULTS Compared with the model group, all serum indices of three TPG groups degrade