武汉大学分子生物学-chapter11-DNA的重组与转座.pptVIP

Chapter 11 Site-Specific Recombination Transposition of DNA OUTLINE Conservative Site-Specific Recombination Biological Roles of Site-Specific Recombination (l phage integration/excision, multimeric genome resolution) Transposition ( concepts, learning from B. McClintock, DNA tranposons. Viral-like retrotransposons/retroviruses, poly-A retrotransposons) Topic 1: Conservative Site-Specific Recombination CSSR can generate three different types of DNA rearrangements Figure 11-3 Figure 11-4 Structures involved in CSSR Topic 2 Biological roles of site-specific recombination Many phage insert their DNA into the host chromosome during infection using this recombination mechanism. Example: l phage Alter gene expression. Example: Salmonella Hin recombinase (the details are not discussed in the class) Maintain the structural integrity of circular DNA molecules during cycles of DNA replication. Example: resolvase that resolves dimer to monomer 2-1 2 l integrase works with IHF and Xis to integrate/excise the phage genome into/from the bacterial chromosome The outcome of l bacteriophage infection of a host bacterium Establishment of the lysogenic state: requires the integration of phage DNA into host chromosome lytic growth is the growth stage of multiplication of the independent phage DNA that requires the excision of the integrated phage DNA from the host genome. Figure 11-2: l genome integration. Recombination always occurs at exactly the same sequence within two recombination sites, one on the phage DNA, and the other on the bacterial DNA. 2-5 Recombinase converts multimeric circular DNA molecules into monomers The chromosomes of most bacteria, plasmids and some viral genomes are circular. During the process of homologous recombination, these circular DNA sometimes form dimers and even multimeric forms, which can be can be converted back into monomer by site specific recombination. Site-specific recombinases also called resolvases catalyze such a process. Topic 3 Tr