婴儿源益生性双歧杆菌的筛选及肠道定殖性研究.pdfVIP

现代食品科技 Modern Food Science and Technology 2016, Vol.32, No.10 婴儿源益生性双歧杆菌的筛选及肠道定殖性研究 韩俊燕，赵国忠，杨波，刘小鸣，张灏，陈卫 （江南大学食品学院，江苏无锡 214122） 摘要：本研究旨在从婴儿粪便中筛选出具有潜在益生特性的双歧杆菌，并探究其肠道定殖情况，为双歧杆菌的产品开发提供优 良的菌株。采用MRS 培养基对样品进行分离纯化，菌株经 F6PPK 检测及 16S rDNA 测序鉴定，之后进行模拟胃肠液、胆盐耐受性、 对食源性致病菌（大肠杆菌、沙门氏菌、单增李斯特菌等）的抑制及对HT-29 细胞的粘附能力测定，将筛选出的菌株进行动物实验， 测定其肠道定殖能力。分离到的27 株双歧杆菌，经分子生物学鉴定为 7 个不同的种：Bifidobacterium longum 、Bifidobacterium breve 、 Bifidobacterium bifidum 、Bifidobacterium pseudocatenulatum 、Bifidobacterium infantis 、Bifidobacterium animalis 和 Bifidobacterium adolescentis。体外实验表明，B. longum A9 、B. breve A4 、B. bifidum B6 、B. longum C6 、B. adolescentis F8 和 B. infantis H6 等具有较 强的潜在益生特性；动物实验表明，B. infantis H6 和 B. longum C6 具有较强的肠道定殖能力。B. longum C6 和 B. infantis H6 有望作为 优良的益生性菌株，应用于双歧杆菌的产品开发。 关键词：益生特性；双歧杆菌；筛选；定殖 文章篇号：1673-9078(2016)10-14-21 DOI: 10.13982/j.mfst.1673-9078.2016.10.003 Screening of Probiotic Bifidobacterium Strains from Infants and Study of Their Intestinal Colonization Ability HAN Jun-yan, ZHAO Guo-zhong, YANG Bo, LIU Xiao-ming, ZHANG Hao, CHEN Wei (School of Food Science and Technology Jiangnan University, Wuxi 214122, China) Abstract: Bifidobacterium strains with potential probiotic properties were screened from infant feces and their colonization ability in the intestine was explored, to provide potential probiotic strains for the development of Bifidobacterium products. De Man, Rogosa, and Sharpe (MRS) medium was used to isolate and purify the samples, and detection of fructose-6-phosphate phosphoketolase (F6PPK) and analysis of 16S rDNA sequences were performed on the strains. The probiotic properties of the strains, including toler