SD大鼠背根神经节细胞新取材和培养方法.docxVIP

华 中 科 技 大 学 硕 士 学 位 论 文 Introduction The neuron of dorsal root ganglion locates in dorsal root ganglion and peripheral nerve system (PNS) of vertebrates; the dorsal root ganglion contains Schwann cells, fibroblasts and satellite cells around neuron, in addition to rich sensory neurons of peripheral nerve system. As the pseudounipolar neuron, DRG neuron is originated from neural crest and formed via migration of pluripotent precursor cell. The axon is rounded by Schwann cell to form the myelin sheath. The dorsal root ganglion cell is a primary sensory neuron that can conduct nervous impulses like touch sensation, pain sensation, warm sensation and so forth. As the link between in vitro and in vivo environment of orga nism and spinal cord, its functions include transmission and regulation of various sensory receptions, and conduction of various injured perceptions, thus it plays an important role in development and maintain of neuropathology. It can conduct nervous impulses such as touch, pain and warm sensations. DRG is important in nervous system as a critical relay station that connects peripheral and central nervous fibers. DRG neuron involves directly in physiopathology of some hereditary and acquired neuropathies. The technology of culture of nervous tissue and cells established by Harrison in 1907, who was the first to culture successfully the implantation plate of neural tube of frog embryo in the lymph clot and observed the activity of neuronal process and its growth cone, so that Cajal’s neuron doctrine is supported. In the last two decades, the nervous culture has become a modern research approach with its modification in methodology and combination with other subjects, and become one of the bridges and links among various subject strategies while there is growing recognition in this field, resulting in effective combination between morphology and functionality, as well as basis and clinic. Its application is expanded while the culture of nervous tissue is