用于本科教学的间接免疫荧光法观察玉簪保卫细胞微管骨架.PDFVIP

中国细胞生物学学报 Chinese Journal of Cell Biology 2017, 39(5): 629–632 DOI: 10.11844/cj cb.2017.05.0352 用于本科教学的间接免疫荧光法观察玉簪 保卫细胞微管骨架 谢冰亮 王语涵 张 琳 王可 卢存福* (北京林业大学生物科学与技术学院, 北京100083) 摘要 细胞骨架的观察是生命科学类专业本科生一个很重要的实验。目前, 在细胞生物学 实验教学中常用考马斯亮蓝染色法观察植物细胞骨架, 但存在诸多不足。该研究选取洋葱、大叶 黄杨、玉簪三种植物材料, 利用间接免疫荧光法对微管进行观察比较。结果表明, 三种植物中, 玉 簪作为一种常见的绿化观赏花卉植物, 取材方便、撕片容易, 其气孔保卫细胞微管骨架免疫荧光图 像显示清晰, 在低温处理后也可观察到微管解聚现象。因此, 玉簪可作为本科生实验教学中利用免 疫荧光法观察微管骨架的一种易得、观察效果好的实验材料。 关键词 玉簪; 保卫细胞; 微管; 低温处理; 实验教学 Observation on Guard Cell Microtubules from Hosta plantaginea by Immunofluorescence Method for Undergraduate Teaching Xie Bingliang, Wang Yuhan, Zhang Lin, Wang Keyue, Lu Cunfu* (College of Biological Science and Biotechnology, Beijing Forestry University, Beijing 100083, China) Abstract Cytoskeleton observation is a significant experiment for undergraduate students majoring in biology. In the experimental teaching of cell biology, coomassie brilliant blue staining is the common method to observe plant cytoskeletons. However, it is not a good method to detect and distinguish different cytoskelons specifically. In present study, we used Allium cepa , Hosta plantaginea , and Buxus megistophylla to observe cell microtubules by immunofluorescence method. The results indicated that Hosta plantaginea , as an ornamental flower plant, is a suitable plant to observe clear fluorescent microtubule images in leaf guard cells. Microtubule depolymerization was detected in samples that treated at 4 °C or –20 °C, indicating that low temperature induced disintegration of the microtubule in guard cells. Therefore, Hosta plantaginea is a proper plant for observing microtubule by immunofluorescence microscopy for undergraduate teaching. Keywords Hosta