Chapter 7 抗原呈递细胞.pptVIP

Chapter 7 抗原呈递细胞; Antigen presenting cell (APC) Definition APC is a cell that displays peptide fragment of protein antigens, in association with MHC molecules, on its surface and activates antigen-specific T cells. In addition to displaying peptide-MHC complexes, APCs must also express costimulatory molecules to activate T lymphocytes optimally. ;T cells do not recognise native antigens;; 第一节 抗原提呈细胞的种类 Professionals APC: Dendritic cells Macrophages B cells;Non-professional APC ： 在某些活性分子刺激下能被诱导表达MHC-II类分子并能处理和提呈抗原的细胞 。包括：???皮细胞；纤维母细胞；上皮及间皮细胞；嗜酸性粒细胞等。抗原提呈能力弱。 广义APC：指能加工处理抗原并以抗原肽-MHC分子复合物的形式提呈抗原信息的所有细胞。 ;2. Surface molecules Immature DC are different from mature DC in phenotype ;DC characteristics: Morphology Expressing high level of MHC II molecule and other co-stimulating molecules. Extravasate to other lympho organ Initiate activation of na?ve T cell ;DC surface markers: 髓系DC： 以CD11ｂ、CD11ｃ、CD13，CD３３为标志 人淋巴样DC： CD11c－/CD123+/BDCA2+/CD45RA+/CD4+ 人DC主要特征性表面标志： CD1α，CD11ｃ，CD83 小鼠DC特异性标志：３３D1，NLDC145 ;Functions of DC 1. Interface of innate and adaptive immunity Immature DC----Unspecific antigen uptake Phagocytosis on the basis of recognition Macropinocytosis Mature DC -----Antigen processing and presentation. 2. Involved in immune tolerance and immune memory. 3. Cytokine secretion. ;Monocytes;B cell; 第二节 抗原的处理与提呈;Antigen processing and presenting;;;;内源性抗原加工，处理及提呈;;外源性抗原加工，处理及提呈 ;;; 脂类抗原的CD1分子提呈CD1分子： 非经典MHC I类分子 提呈抗原：提呈糖脂或脂类抗原供CD1限制性 T细胞识别 CD1限制性T细胞：CD4-CD8-T; TCRγδT 细胞，NKT细胞等 CD1抗原提呈意义：在机体抗微生物感染和对 脂类抗原的应答中起重要作用