细胞实验技术-课堂PPT-第八讲.pptVIP

Primary cell culture Methods in Cell Biology Cells that are cultured directly from a subject are known as primary cells. The cells can be removed from the tissue directly and disaggregated by enzymatic or mechanical means before cultivation. Primary culture refers to the stage of the culture after the cells are isolated from the tissue and proliferated under the appropriate conditions until they occupy all of the available substrate. Methods Trypsin-digestion method, Explant culture Primary??culture Material：postnatal?day?1 Sprague?Dawley?rat?pups Reagents: PBS , DMEM, Supplemented D