何首乌毛状根对外源化合物的生物转化研究以及贯叶连翘中化学成分的分离纯化（可编辑）.docVIP

何首乌毛状根对外源化合物的生物转化研究以及贯叶连翘中化学成分的分离纯化 暨南大学硕士学位论文 中文摘要 该论文由两部分组成。第一部分为何首乌毛状根对外源化合物的生物转化 研究;第二部分为贯叶连翘Hypericum perforatum L.中化学成分的研究。 第一部分研究了何首乌毛状根对呋喃橐吾酮的生物转化。何首乌毛状根生物 转化体系预培养10 d后,加入乙醇溶解的外源底物呋喃橐吾酮,共培养一段时 间后终止转化。通过TLC手段检测培养物和培养基中的产物,并利用柱层析法分 离得到两个转化产物和,通过理化性质和谱学方法鉴定其结构分别为: 3-oxo-eremophila-1,7 11 -dien-12,8-olide和3-oxo-8-hydroxy-eremophila-1,7 11-dien-12,8-olide。 第二部分为贯叶连翘中化学成分的研究。作者利用大孔树脂、硅胶柱层析等 多种现代色谱分离技术从贯叶连翘中分离得到 6个化合物,并运用波谱学方法, 结合化学定性鉴别和物理常数测定等方法,对其中 3个化合物进行了结构鉴定。 分别为:金丝桃素hypericin、β-谷甾醇 β-sitosterol 和胡萝卜苷daucosterol。 关键词:何首乌毛状根;生物转化;呋喃橐吾酮;贯叶连翘;金丝桃素;分离鉴 定 1 Create PDF with GO2PDF for free, if you wish to remove this line, click here to buy Virtual PDF Printer暨南大学硕士学位论文 Abstract The paper is composed of two parts. The first one is the research on biotransformation of xenobiotics by transgenic hairy roots of Polygonum multiforum. The second is about the isolation and purification of chemical compounds in Hypericum perforatum L. In the first part, biotransformation of furanoligularenone in the hairy roots of P. multiforum was studied. After pre-incubation of the hairy roots of P. multiforum for ten days, the substrate was added into the hairy roots system. And then they were co-cultured for some days. Two transformed products, 3-oxo-eremophila-1,711- di-en-12,8-olide and 3-oxo-8-hydroxy-eremophila-1,711-di-en-12,8-olide were isolated from the culture and medium. The second chapter of the thesis is about the chemical constituents from H. perforatum. Six compounds were isolated from the plants through the mode rn chromatographic, such as macroporous resin, silica gel column chromatograp hy. Three compounds, hypericin,β-sitosterol and daucosterol were identified by 1 13 physical-chemical properties and spectra data H-NMR, C-NMR, MS. Key words:Transgenic Polygonum multiforum; Hairy roots; Biotransformation; furanoligularenone, Hypericum perforatum; chemical constituents; Isolation and structural elucidation 2 Create PDF with GO2PDF for free, if you