Anne切xin A4对结直肠癌EMT及侵袭转移的影响.docx

优秀毕业论文 精品参考文献资料 具有统计学意义(P0 具有统计学意义(P0．05)，而E-candherin的表达水平明显升高，差 异具有统计学意义(P0．001)； 5．siRNA技术干扰结直肠癌HT29细胞的Annexin A4表达后，Western Blot法检测结果显示siA4组结直肠癌HT29细胞的上皮细胞标志物 E-cadherin的蛋白表达水平明显高于siNC组，而间充质细胞标志物 N-caderin的蛋白水平明显降低； 6．siRNA技术干扰结直肠癌HT29细胞的Annexin A4表达后，MTT实 验检测结果显示siA4组结直肠癌HT29细胞增殖速度较siNC组明显 降低((P0．01)，细胞克隆形成实验检测结果显示siA4组细胞克隆 形成数较siNC组明显减少(PO．001)。 7．siRNA技术干扰‘结直肠癌HT29细胞的Annexin A4表达后， Transwell基质胶侵袭实验检测结果显示siA4组结直肠癌HT29细胞 侵袭小室的细胞数目较siNC组明显减少((P0．01)，划痕愈合实验 检测结果显示-siA4组细胞的迁移能力较siNC组明显减弱； 结论： 1．结直肠癌中Annexin A4的表达水平与EMT关键分子密切相 关，可作为结直肠癌侵袭转移的潜在分子标志物； 2．siRNA干扰Annexin A4的表达下调能有效抑制结直肠癌细胞 系HT29的体外生长、侵袭和迁移能力。 关键词：结、直肠肿瘤：上皮～间质转化；膜联蛋白A4；侵袭；迁移； 万方数据 IV 万方数据 ABSTRACTBackground ABSTRACT Background and 0bjective： Colorectal cancer is a common malignant tumor of digestive tract，and the inciden．c．e rate is increasing in recent years．The incidence of colorectal cancer IS a complex process involving multiple genes and multiple steps， SO its exact pathogenesis is still not clear．Annexins are a superfamily binding with negatively charged phos—pholipid membranes in a Ca2+-dependent manner．Annexins participate in cytoskeletal organization， exocytosis，endocytosis，and apoptosis，they also play important roles in proliferation，invasion，metastasis，and drug resistance of cancer．Annexin A4(Anxa4)is one of the Ca2+-regulated and phospholipid．binding annexin superfamily proteins． Recently，the expression and relocalization of Anxa4 have been reported that it is associated with the development of metastasis in some invasive malignancies suggesting a role for this protein in regulates epithelial cell migration and invasion．However，the specific mechanism is not clear．In this study，we employ siRNA-mediated gene silencing of Annexin A4 to explore the effect of Annexin A4一si RNA on the expression of Annexin A4 and epithelial—mesenchymal transition—related gene and to evaluate its effects on the biological behaviors(proliferation，invasion，and migration)of colorectal carcinoma cells，further enr