AMPKα2在二甲双胍抑制香烟暴露阿下肺动脉平滑肌细胞表型转化中的作用.docx

优秀毕业论文 精品参考文献资料 第三军医大学硕士学位论文NO 第三军医大学硕士学位论文 NO Nitric Oxide 一氧化氮 PDGF Platelet derived growth factor 血小板衍生生长因子 PVDF Polyvinylidene difluoride membrane 聚偏氟乙烯膜 Ccr2 C-C chemokine receptor 2 CC趋化因子受体2 NF．r．B Nuclear Factor．r,B 核因子．rd3 COX一2 Cyclooxygenase-2 环氧合酶．2 g gram 克 h hour 小时 mln minute 分钟 second 秒 L liter 升 ml milliliter 毫升 肛l microliter 微升 mg milligram 毫克 mol mole 摩尔 mM millimole／L 毫摩尔／升 nM micromole／L 微摩尔／升 rpm revolution per minute 辕{食 PAGE Polyacrylamide gel electrophoresis 聚丙烯酰胺凝胶电泳 SDS Sodium dodecyl sulfate 十二烷基硫酸钠 TBS Tris buffer saline Tris缓冲液 WB Wbstern Blot 蛋白印迹法 TEMED Tetramethylethylenediamine 四甲基乙二胺 EDTA Ethylenediaminetera 乙二胺四乙酸 2 万方数据 第三军医大学硕士学位论文Metformin 第三军医大学硕士学位论文 Metformin inhibits phenotypic switch of pulmonary arterial smooth muscle cells induced by cigarette smoking extract in AMPKa2··dependent pathway Abstract Background： Pulmonary hypertension(PH)，as one of the major complications of chronic obstructive pulmonary disease(COPD)，directly affects the quality of life and prognosis of patients with COPD．Cigarette smoking is the main risk factor for COPD．Much evidence from clinical samples and animal model has shown that pulmonary vascular remodeling was found in mild-moderate COPD patients with cigarette smoking even in smokers with normal lung function without hypoxia．High oxidative stress and chronic inflammation caused by cigarette exposure in COPD patients play an important role in the formation of pulmonary vascular remodeling and PH． In recent years，AMP—activated protein kinase(AMPK)，which regulates the balance of cellular energy metabolism，has attracted much attention important target for regulating oxidative stress and inflammation．In patients with diabetes and obesity,low AMPK activity in vivo is closely related to the high oxidative stress status，chronic inflammation and the higher incidence of cardiovascular diseases．The activation of AMPK is important for maintaining the normal structure and function of blood vessels．Sev