BD流式全面介绍课件.ppt

BD流式全面介绍;流式细胞术;流式细胞仪系统;Injector Tip;流式细胞仪的光信号;1. 散射光信号;前向角散射光 ——FSC;侧向角散射光——SSC;散射光;散点图——Dot Plot;Review Question;2. 荧光信号;荧光检测器;Two-Color Cell Analysis;现代流式细胞仪包括;液流系统;Sample Flow in Optical Cuvette;Review Question;Optics;Optical Filters;FACSCalibur光路图;Optics;Electronics;Creation of a Voltage Pulse - Analog Signal;Quantification of a Voltage Pulse;Effect of the Instrument Controls on the Data;Review;Review Questions;Review Questions;Review Questions;样本处理;细胞悬液的制备;步骤一： 选择合适的荧光染料;荧光产生过程;Propidium Iodide;Fluorescein (FITC);Excitation;Allophycocyanin (APC);FITC和PE荧光光谱;补偿调节前后对比;步骤二： 染色;直接染色;间接染色;Avidin-Biotin method I;步骤三： 数据收集和分析;仪器设置调节;关于同型对照;数据分析;如何设定阴性与阳性的界限;细胞结构 细胞大小 细胞粒度 细胞表面面积 核浆比例 DNA含量与细胞周期 RNA含量 蛋白质含量 染色体分析;流式细胞仪的临床应用;流式细胞仪的科研应用;流式细胞仪的遗传学应用;What can Flow Cytometer tell us about a cell?;细胞增殖（细胞周期）;DNA 探针; Nucleic acid Probes;G2;A typical DNA Histogram;红色为正常二倍体细胞；黄色为异倍体细胞; 增殖相关基因; BrdUrd Incorporation; BrdUrd Incorporation; 细胞分裂的分析 ;Tracing Dye(CFSE); Apoptotic Cell Death -- a genetically encoded cell death program, which is morphologically, biochemically and molecularly distinct from necrosis;Flow cytometry of apoptotic cell death;Flow cytometry of apoptotic cell death;Flow cytometry of apoptotic cell death;Flow cytometry of apoptotic cell death;Flow cytometry of apoptotic cell death;Flow cytometry of apoptotic cell death Phospholipid redistribution;Flow cytometry of apoptotic cell death;Flow cytometry of apoptotic cell death;Flow Cytometry of Apoptotic Cells; An Integrated Approach  to Cell Immunology;Immune Function assay;淋巴细胞亚群分析;淋巴细胞亚群分析;Absolute Counts -- Cells per mL;抗原特异性T细胞;MHC: Class I and Class II Gene Products;MHC/TCR Signaling;二聚体可溶性MHC类似物与四聚体的比较;;Treg细胞的检测;;Immune Function Assays -- A Powerful Research Tool for Answering Basic Biological Questions in...;Traditional Cytokine Flow Cytometry;细胞因子检测;T细胞的细胞因子分型;细胞因子;Biological Variation Among CMV-seropositive Donors in