冷却猪肉中单增李斯特氏菌生长动力学模型的建立与-食品科学.PDFVIP

234 2009, Vol. 30, No. 21 食品科学 ※生物工程 李苗云，张秋会，赵改名，黄现青，高晓平，柳艳霞，杨欢欢 (河南农业大学食品科学技术学院，河南郑州 450002) 为建立冷却猪肉中单增李斯特氏菌的生长动力学模型，把单增李斯特氏菌接种到无污染的冷却猪肉中，分 别放置于2 、15、28 ℃条件下贮藏，分别测定其在不同贮藏时间的菌数。应用修正的Gompertz 方程描述单增李 斯特氏菌在2 、15、28 ℃下的生长动态，建立不同温度下其在冷却猪肉中的生长曲线和模型。温度对最大比生长 速率( μmax)和延滞时间( λ) 的影响，采用平方根模型在0 ～30 ℃范围内呈现较好的线性关系，R 2 分别为0.7164 和 0.9717 。模型残差值的绝对值均小于0.03 ，在“零”上下浮动，表明该模型描述的温度与μmax 和λ( 的关系是完 全可信的，说明用平方根模型能很好的描述不同温度对单增李斯特氏菌生长的影响。 冷却猪肉；单增李斯特氏菌；生长动力学模型 Establishment and Evaluation of Growth Kinetics Model for L. monocytogenes in Chilled Pork LI Miao-yun ，ZHANG Qiu-hui ，ZHAO Gai-ming ，HUANG Xian-qing ，GAO Xiao-ping ，LIU Yan-xia ，YANG Huan-huan (College of Food Science and Technology, Henan Agricultural University, Zhengzhou 450002, China) Abstract ：In order to establish growth kinetics models for L. monocytogenes in chilled pork, L. monocytogenes were inoculated to contamination-free longissimus muscles to monitor the growth of this species of bacteria during storage. Samples were placed at 2, 15 and 28 ℃ for storage, and total L. Monocytogenes count was determined during different storage periods. Growth kinetics models for L. monocytogenes were developed at various temperatures using Gompertz equation. The relationship between temperature and μmax (maximum specific growth rate) or λ(lag phase) of L. monocytogenes growth in temperature range of 0 －30 ℃ revealed a good linearity evaluated by the square root model with correlation coefficients of 0.716 and 0.972, respectively. The absolute values of model residuals were less than 0.03 and fluctuated around zero, which revealed an absolute reliability of this model. Therefore, this square root model can describle the effect of temperature on growth of L. monocytogenes. Key words ：chilled pork ；L.