昆明小鼠物体识别模型的建立及其在检测化学药物对记忆损伤中的应用.pdf

动 物 学 研 究 2007，Oct. 28 (5): 497－500 CN 53-1040/Q ISSN 0254-5853 Zoological Research 昆明小鼠物体识别模型的建立及其在检测 化学药物对记忆损伤中的应用 * 摘要：本研究的主要目的是建立昆明小鼠物体识别模型并评价该模型在安全药理学研究中的潜在应用价值。 研究了昆明小鼠物体识别记忆随时间而减弱的特性，在训练结束后 4 h 或 1 h，检测昆明小鼠的物体识别记忆，并 评价了东莨胆碱对昆明小鼠物体识别记忆的影响。结果表明：1 h 间隔组昆明小鼠熟悉期探究物体的时间差和测 试期探究物体的时间差存在显著差异(P0.05) ，昆明小鼠在训练结束后 1 h 记忆保持良好，可以进行物体识别；东 莨胆碱组昆明小鼠熟悉期探究物体的时间差和测试期探究物体的时间差比较(P0.05) ，没有显著性差异。因此， 东莨胆碱损伤了昆明小鼠的物体识别记忆。用昆明小鼠建立的物体识别模型具有简单、快速、可靠等特点，在安 全药理学研究中可用于检测化学药物对记忆的损伤。 关键词：昆明小鼠；物体识别；东莨胆碱；安全药理学 中图分类号：R964 文献标识码：A 文章编号：0254-5853 （2007 ）05-0497-04 Founding of Object Recognition Learning Paradigm in KM Mice and Its Application in Detecting Memory Impaired Properties of Compounds * CHE Yi , CUI Yong-hua, ZHU Yu-fang, XUE Zhi-mou (School of Life Science, Suzhou University, Suzhou 215006, China) Abstract: The aim of the present study is to found the object recognition learning paradigm in KM mice and evaluate its potential application in safety pharmacology. The time dependent decay of object recognition memory was characterized in KM mice. Memory retention was tested 4h or 1h after the training and the impairment of scopolamine on the object recognition memory was assessed. The results showed a great difference between the time of exploring the two objects in the choice phase and the time of spending for novel object and for familiar object in one hour intertrial interval group, P0.05. No difference appeared between the time of exploring the two objects in the choice phase and the time of spending for novel o