粮食及果蔬中叶酸含量分析-食品科学.pdf

290 2014, Vol.35, No.24 食品科学 ※分析检测 粮食及果蔬中叶酸含量分析 1 1,2,* 邵丽华 ，王 莉 （1.山西师范大学生命科学学院，山西 临汾 041004 ；2. 山西师范大学现代文理学院生物系，山西 临汾 041004 ） 摘  要：对日常食用的主要粮食及果蔬的叶酸含量进行测定与评价，为人体科学合理补充叶酸提供理论依据。在山 西省各农户及市场搜集11 种主要粮食、17 种常见蔬菜、8 种常见水果。粮食样品研磨全部过100 目筛子备用，市售 新鲜果蔬样品将可食用部分随机多点采样，切碎混合。食物叶酸用磷酸二氢钾溶液恒温水浴浸提，加苯胺处理过的 活性炭吸附，用体积分数3%氨-70%乙醇洗脱，采用高锰酸钾氧化-间接荧光法测定。结果显示，主要食物叶酸含量 存在差异，叶酸含量较高的粮食种类有：大豆、花生、绿豆、小米、燕麦、玉米和苦荞，含量均在1.56 μg/g （以干 质量计）以上；含量较高的蔬菜有：菠菜、油菜、香菇、油麦菜、生菜、卷心菜、青椒、芹菜、白菜和南瓜，含量 均在1.53 μg/g （以湿质量计）以上；人们日常食用的水果叶酸含量均在1.74 μg/g （以湿质量计）以上；只要合理调 整膳食结构，粗细搭配，多摄食蔬菜水果，就有望通过食疗来补充人体所缺乏的叶酸。 关键词：粮食；水果；蔬菜；叶酸含量 Analysis of Folic acid Contents in Main Grain Crops, Fruits and Vegetables 1 1,2,* SHAO Li-hua , WANG Li (1. College of Life Science, Shanxi Normal University, Linfen 041004, China; 2. Department of Biology, College of Modern Arts and Sciences, Shanxi Normal University, Linfen 041004, China) Abstract: In this study, the contents of folic acid in main grain crops, fruits and vegetables were determined and evaluated ’ to provide a theoretical basis for reasonable supplementation of folic acid for people s daily consumption. A total of 38 samples, including 11 kinds of main grain crops, 17 kinds of common vegetables and 8 kinds of common fruits, were collected from some households and local markets in Shanxi Province. Grain samples were prepared by grinding and s