分子微生物生态学手册.doc

Molecular Microbial Ecology Manual 1. Simplified protocols for the preparation of genomic DNA from bacterial cultures 2. Extraction of ribosomal RNA from microbial cultures 3. Extraction of microbial DNA from aquatic sources: Marine environments 4. Extraction of microbial DNA from aquatic sources: Freshwater 5. Methods for extracting DNA from microbial mats and cultivated micro-organisms: high molecular weight DNA from French press lysis 6. Extraction of microbial DNA from aquatic sediments 7. Extraction of microbial RNA from aquatic sources: Marine environments 8. Extraction of total RNA and DNA from bacterioplankton 9. Methods for extracting RNA or ribosomes from microbial mats and cultivated microorganisms 10. Cell extraction method 11. DNA and RNA extraction from soil 12. Rapid simultaneous extraction of DNA and RNA from bulk and rhizosphere soil 13. Direct Extraction of Fungal DNA from Soil 14. Purification of microbial genes from soil and rhizosphere by magnetic capture hybridization and subsequent amplification of target genes by PCR 15. Direct ribosome isolation from soil 16. DNA Extraction from Actinorhizal Nodules 17. Quantification of nucleic acids 18. Quantification of nucleic acids from aquatic environments by using green-fluorescent dyes and microtiter plates 19. Degradation and turnover of extracellular DNA in marine sediments 20. Incorporation of thymidine into DNA of soil bacteria 21. Preparation of radioactive probes 22. Detection of Nucleic Acids by Chemiluminescence 23. Parameters of nucleic acid hybridization experiments 24. Detection and quantification of microbial DNA sequences in soil by Southern- and dot/slot blot hybridization 25. Detection of microbial DNA sequences by colony hybridization 26. Polymerase chain reaction analysis of soil microbial DNA 27. Detection of microbial nucleic acids by polymerase chain reaction in aquatic samples 28. Isolation and detection of bacterial DNA sequences in dairy products 2