外源基因转化在小麦育种中的应用-河南农业科学.pdf

DOI :10.15933/j.cnki.1004 -3268.2004.04.003 2004 年第4 期 1 1 2 冯伟森, 黄群策 , 姜秋会 (1 , 450052;2 ) :远缘杂交是创造生物新物种和新种质以及转移遗传物质的有效途径, 在理论和实践上都 有 要意义。外源DNA 导入技术以DNA 片断杂交技术假说为理论依据, 直接将带有目的性 状的供体遗传物质(总DNA)或目的基因导入受体植物, 创造大量的变异材料, 通过筛选获得 具有目的性状的后代, 达到改良品种的目的。笔者综述了近年来外源基因导入小麦的研究概 况, 阐述了外源基因导入的技术以及检测方法, 分析了外源基因转化在小麦育种中的发展前 景。 :外源基因;远缘杂交;小麦;育种 :S512 　　:A　　:1004-3268(2004)04-0009-07 Application of the Exogenous Gene ransformation in Wheat Breeding 1 1 2 FENG Wei-sen , HUANG Qun-ce ,JIANG Qiu-hui (1 Henan Provincial Key Laboratory of Ion Beam Bioengineering Zhengzhou University, Zhengzhou 450052, China;2 College of Life Science, Qinghai Normal University) Abstract:Distant cross is an effective method creating new specses, new germplasm and transfer- ing exogenous gene in biological bodies.he technique for introducing exogeneous gene between plants, based on the pothesisof DNA fragments crossing, is used to directly introduce total DNA or target gene into plants, so as to produce a large quanitity of variant materials and obtain progeny with target gene by means of screening, which can improve variety.his report summarized the researching status of wheat exogenous gene transformation in recent years. he prospects of its applications in wheat breeding field was also analysed. Key words:Exogenous gene;Distant cross;Wheat;Breeding 　　20 , [1] 。 , , , 。 , , 、、、、 。 、、、、 1　DNA 、、