水稻单叶独立转绿型黄化突变体grc2的鉴定与基因精细定位-作物学报.pdf

作物学报 ACTA AGRONOMICA SINICA 2015, 41(6): 831837 / ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2015.00831 水稻单叶独立转绿型黄化突变体grc2 的鉴定与基因精细定位 谭炎宁1,2,** 孙学武2,** 袁定阳2 孙志忠2 余 东2 何 强2 段美娟2,* 邓华凤1,2,* 袁隆平1,2,* 1 , 410128; 2 , 410125 60 : grc2 Co-γ T98B grc2 , 10 d , T98B , grc2 b , , grc2 , grc2 1 ; grc2/Nipponbare F2 960 , grc2 STS S254 S258 31 kb , 5 grc2 : ; ; ; Identification and Fine Mapping of Green-Revertible Chlorina Gene grc2 in Rice (Oryza sativa L.) 1,2,** 2,** 2 2 2 2 TAN Yan-Ning , SUN Xue-Wu , YUAN Ding-Yang , SUN Zhi-Zhong , YU Dong , HE Qiang , DUAN Mei-Juan2,* , DENG Hua-Feng1,2,*, and YUAN Long-Ping1,2,* 1 College of Agronomy, Hunan Agricultural University, Changsha 410128, China; 2 State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Changsha 410125, China Abstract: Green revertible leaf-color mutants are basical materials for studying the mechanism of chloroplast differentiation and development. We have obtained a green-revertible chlorina mutant named grc2 with every leaf greening independently, from an indica maintainer line T98B treated by 60Co-γ radiation. Each leaf of grc2 is initially chlorotic, and then turns green after growing about 10 days. The mutant grc2 showed a new pattern of virescence which refreshed green regardless of its plant growth stage. Compared with the wild type T98