正交试验优选藏药独一味提取工艺Δ.PDF

正交试验优选藏药独一味提取工艺Δ ＊ # 邱建国 ，李茂星，张泉龙，尉丽力，贾正平，张汝学，周 珺，邱宜农，王春英，陶 锐（兰州军区兰州总医院药材 科/全军高原环境损伤防治重点实验室，兰州 730050） 中图分类号 R283.6 文献标志码 A 文章编号 1001-0408（2014）19-1760-04 DOI 10.6039/j.issn. 1001-0408.2014.19.10 摘 要 目的：优选独一味的提取工艺。方法：以总环烯醚萜苷、总黄酮质量浓度和干浸膏得率为指标，以加水量、提取次数、提取 时间为考察因素，采用正交试验优选独一味的提取工艺。同时采用冷藏除去杂质、低温减压干燥等工艺制备干浸膏。结果：优选 工艺为加26倍量水量（初次补加4倍量的水），提取3次，每次1.5h ，水提取物在2～4 ℃下冷藏除去杂质，80 ℃减压干燥；该工艺 条件下，总环烯醚萜苷质量浓度为45.35μg/ml ，总黄酮质量浓度为37.89μg/ml ，干浸膏得率为39.29％。结论：所选工艺稳定、可 靠，有助于提高独一味水提取工艺及以水提物为原料生产的相关制剂的质量。 关键词 独一味；总黄酮；总环烯醚萜苷；干浸膏得率；提取工艺；正交试验 Optimization of the Extraction Process of Tibetan Medicine Lamiophlomis rotata by Orthogonal Test QIU Jian-guo ，LI Mao-xing ，ZHANG Quan-long ，WEI Li-li ，JIA Zheng-ping ，ZHANG Ru-xue ，ZHOU Jun ，QIU Yi-nong ，WANG Chun-ying ，TAO Rui （Dept. of Pharmacy ，Lanzhou General Hospital of PLA ，Key Lab of Pre- vention and Cure for Plateau Environment Damage of PLA ，Lanzhou 730050，China ） ABSTRACT OBJECTIVE ：To optimize the extraction process of Lamiophlomis rotata . METHODS ：The optimum extraction pro- cess was selected by orthogonal test with the yield of total iridoid glycoside ，total flavonoids and dry extract as index using the amount of water ，extraction times and extraction time as factor. The dry extract was prepared by cold storage ，dust removal ，dried under reduced pressure and low temperature. RESULTS ：The optimum technology was as follows ：adding 26-folds of water （4-folds of water at the first time），extracting for 3times ，1.5h per time ，removing impurity at 2-4 ℃，reducing pressure and dry- ing at 80 ℃；under the condition ，the concentration of total iridoid glycoside was 45.35 μg/ml ，the concentration of total flavo- noids was 37.89 μg/ml ，and the yield of dry extract was 39.29％. CONCLUSIONS ：The optimum technology is stable and reli- able ，contributes