Structural basis of pre-mRNA splicing前信使RNA剪接的结构基础.pptxVIP

Structural basis ofpre-mRNA splicing;Background;Introduction;Organization of The RNA components;Fig. 2. Structures of individual RNA components in the spliceosome. (A) An overall illustration of the RNA architecture.The catalytic center—which comprises the ISL of U6 snRNA, Helix I of the U2/U6 duplex, Loop I of U5 snRNA, and the Mg2+ ions—is circled.The disordered RNA sequences are indicated with dotted lines. (B) Structure of U5 snRNA. An illustration of the U5 snRNA structure is shown at right, with its secondary structural elements indicated at left.The sequences in light color are invisible because of their intrinsic flexibility. (C) Overall base-pairing interactions among U2 snRNA, U6 snRNA, and the intron lariat. Base-pairing interactions in the RNA triplex between A41-G40, U68 and A47-G48-C49 are identified with solid lines. (D) Structure of U6 snRNA.The intron lariat is shown here to indicate its relative position to the active site. (E) Structure of U2 snRNA.The view in (D) and (E) is the same as that in (A).;Fig. 3. The catalytic center and the active site of the spliceosome. (A) An overall illustration of the catalytic center of the spliceosome. Three perpendicular views are shown.The intron lariat, Helix II of the U2/U6 duplex, and Stem I of U5 snRNA are included so as to indicate the relative orientation. (B) A close-up view on the catalytic center. Helix I of the U2/U6 duplex is placed to the left of the two catalytic Mg2+ ions, and the ISL of U6 snRNA is close to Loop I of U5 snRNA. (C) A close-up view on the active site. Among the two catalytic Mg2+ ions, the first (M1) is coordinated by phosphate groups from U68,G66, and G48 of U6 snRNA, and the second (M2) is bound by phosphates from U68, G48, and A47. A47 and G48 are part of the RNA triplex;U5 snRNA and U5 snRNP;Placement of snRNA by protein components ;Putative location of the 5′-exon;Comparision with the groupⅡB self-splicing intron; Thanks For Listening!