生物信息学相关操作.docxVIP

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生物信息学相关操作.docx

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1 在地址栏填入 /protparam/，跳转到如下： ProtParam tool ProtParam?( References?/? Documentation) is a tool which allows the computation of various physical and chemical parameters for a given protein stored in? Swiss-Prot or TrEMBL?or for a user entered sequence. The computed parameters include the molecular weight, theoretical pI, amino acid composition, atomic composition, extinction coefficient, estimated half-life, instability index, aliphatic index and grand average of hydropathicity (GRAVY) ( Disclaimer). Please note that you may only fill out?one?of the following fields at a time. Enter a Swiss-Prot/TrEMBL accession number (AC) (for example?P05130) or a sequence identifier (ID) (for example?KPC1_DROME):Or you can paste your own sequence in the box below: ? 在上方小框内填入p05130，点击computer parameters，跳转到如下： ProtParam Selection of endpoints on the sequence KPC1_DROME?(P05130) Protein kinase C, brain isozyme (EC 3) (PKC) (dPKC53E(BR))?Drosophila melanogaster (Fruit fly).? Please select one of the following features by clicking on a pair of endpoints, and the computation will be carried out for the corresponding sequence fragment. By default, the complete sequence is used.Note:?Only the features corresponding to subsequences of at least 5 residues are highlighted. FT CHAIN 1-679 Protein kinase C, brain isozyme. FT DOMAIN 191-278 C2. FT DOMAIN 350-608 Protein kinase. FT DOMAIN 609-679 AGC-kinase C-terminal. FT ZN_FING 45-104 Phorbol-ester/DAG-type 1. FT ZN_FING 119-169 Phorbol-ester/DAG-type 2. FT NP_BIND 356-364 ATP (By similarity). Or, if you wish to select a different sequence fragment (at least 5 amino acids long), you can enter the desired endpoints on the sequence here (by default, the computation will be carried out for the complete sequence). N-terminal:?C-terminal:? The sequence KPC1_DROME consists of 679 amino acids. ?? 默