结构生物学NMR资料教程.pptVIP

第三章 核磁共振波谱学;X射线晶体学和NMR波谱学测定的最大结构的比较;;强度为900mHz的Vrian公司的NMR波谱仪;3.2 NMR的基本原理;;;;;;;;3.3 多维NMR;;;3.4 多维NMR确定蛋白质溶液三维结构的基本原理;;;3.5 蛋白质溶液三维结构的计算;;;;3.6 多维NMR方法测定蛋白质溶液三维结构的实例胰岛素的NMR结构研究;B链羧端去七肽(B24～B30)胰岛素： 一种新晶型的结构;3.7 蛋白质折叠的NMR研究; The Sweetest Puzzle Getting a protein structure using NMR is a lot of fun, says Chele DeRider, a graduate student at the University of Wisconsin-Madison. Youre given all these pieces to a puzzle and you have to use a set of rules, common sense, and intuitive thinking to put the pieces together. And when you do, you have a protein structure. DeRider is working at UW-Madisons national NMR facility. She is refining the structure of brazzein, a small, sweet protein. Most sweet-tasting molecules are sugars, not proteins; so brazzein is quite unusual. It also has other remarkable properties that make it attractive as a sugar substitute. It is 2,000 times sweeter than table sugar —with many fewer calories. And, unlike aspartame (NutraSweet?), it stays sweet even after 2 hours at nearly boiling temperatures. In addition to its potential impact in the multimillion-dollar market of sugar substitutes, brazzein may teach scientists how we perceive some substances as sweet. Researchers know which amino acids in brazzein are responsible for its taste— changing a single one can either enhance or eliminate this flavor —but they are still investigating how these amino acids react with tongue cells to trigger a sensation of sweetness. Getting a protein structure using NMR is a lot of fun ... You start out with just dots on a page and you end up with a protein structure. ;The plum-sized berries of this African plant contains brazzein, a small, sweet protein. Photo Credit: H. M. Hadik ;The fruit of Pentadiplandra brazzeana Baillon contains a small, sweet-tasting protein named brazzein. The structure of brazzein in solution was determined by proton nuclear magnetic resonance spectroscopy at pH 5.2 and 22 C.{Caldwell, Abildgaard, et al. 1998 ID: 24