复制第五讲教学提纲.pptVIP

Formation of pre-Replicative complex;;Eukaryotic initiation complex;A/T;;ORC uses different strategies for binding DNA in different species;Pre-RC formation;The Xenopus Cdc6 Protein Is Essential for the Initiation of a Single Round of DNA Replication in Cell-Free Extracts Made from Xenopus egg extracts;Orc is required to load CDC6; CDC6 is required to load MCM;Immunostaining for CDC6 can pick out proliferating cells in Pap smears;Pre-RC formation;;Propidium iodide staining;Chromatin immunoprecipitation (ChIP) tests whether a given DNA sequence is bound by a protein in vivo;;MCM homolog from M.