响应面法优化超临界萃取花生壳中木犀草素工艺.docxVIP

响应面法优化超临界萃取花生壳中 木犀草素工艺 王胜利1,2，任 云1，王 博1 (1.河北邯郸学院化学系，邯郸 056005； 2.邯郸市有机小分子材料重点实验室，邯郸 056005) 摘要：对超临界CO2萃取花生壳中木犀草素的工艺条件进行响应曲面法优化探讨。建立了有关 木犀草素提取二次多项式回归模型，确定最佳工艺条件为：萃取压力为33.6 MPa，分离压力为 11.72 MPa，CO2流速为6.38 L/h；在此条件下，木犀草素提取的综合评价指标Z%(为木犀草素含 量(X%)与得率(Y%)的算术平均值)为9.30%，与预测结果吻合。并与传统溶剂提取法进行比较， 超临界CO2萃取工艺的综合评价指标Z%为乙醇回流法的2.4倍，木犀草素提取率由51.2%提高到 87.8%。 关键词：木犀草素；花生壳；超临界CO2萃取；响应面法 中图分类号：O 652.62 文献标志码：A 文章编号：1005-9989(2014)10-0262-05 DOI:10.13684/j.cnki.spkj.2014.10.057 Optimization of supercritical carbon dioxide extraction of luteolin from peanut hull with response surface methodology WANG Sheng-li1,2, REN Yun1, WANG Bo1 (1.Department of Chemistry, Handan College, Handan 056005; 2.Handan Key Laboratory of organic small molecule materials, Handan 056005) Abstract: The supercritical fluid CO2 extraction parameters for extracting luteolin from peanut hull in response surface methodology experimental design were studied. A regression equation with high reliability was obtained, and the technology parameters were optimized by the equation. The optimized parameters for extracting luteolin from peanut hull with supercritical fluid CO2 are extraction pressure 33.6 MPa, separation pressure 11.72 MPa, CO2 flow rate 6.38 L/h. Under the optimized conditions, The extraction comprehensive evaluation index Z% (Z% is the arithmetic average of the content (X%) of luteolinand yield (Y%)) was 9.30%. The results showed the model fitted well the experimental data and the model was feasible. Compared with traditional solvent extraction method, the comprehensive evaluation index of supercritical CO2 extraction technology is 2.4 times of ethanol refluxing. The luteolin extraction rate was increased from 51.2% to 87.8%. Key words: luteolin; peanut hull; supercritical CO2 extraction; response surface methodology 收稿日期：2014-04-01 基金项目：河北省科技攻关计划项目；邯郸学院博、硕士启动项目(2005004)。 作者简介：王胜利(1968—)，女，硕士，副教授，研究方向为天然植物抗氧化剂提取及性能研究。 花生属豆科一年生草本油料植物，在世界 范围内种植广泛。目前，主要开发利用仁和衣作 ??(%)= N???? N??