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2013 32 6 CHEMICAL INDUSTRY AND ENGINEERING PROGRESS 1205
610065
610065
ABTS DPPH
CAA
TS 201 A 100066132013
DOI 10.3969/j.issn.1000-6613.2013.06.001
Common methods of antioxidant activity evaluation for natural
products A review
ZENG WeicaiSHI Bi
Key Laboratory of Leather Chemistry and Engineering of Ministry of EducationSichuan UniversityChengdu
610065SichuanChina Department of Biomass and Leather EngineeringSichuan UniversityChengdu 610065
SichuanChina
Abstract Evaluation of antioxidant activity is important for the investigation of natural productsand
is the useful guide for their application in chemical industry. In the present studysome typical
analytical methods for evaluation of the antioxidant activity of natural products are reviewed in terms
of their chemical mechanisms and common procedures. The in vitro methodssuch as free radical
(ABTSDPPHsuperoxide and hydroxyl free radical) scavenginglipid peroxidationreducing power
and fat oxidation assays are introduced. Meanwhilethe cellular antioxidant activity (CAA) experiment
based on determination of the vitality of cells is also described. Furthermorethe testing procedures and
factors of in vivo method in animals are given. This review might be useful for the related research of
natural products.
Key words natural products antioxidant activity analytical
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