微卫星DNA和AFLP标记在水稻.ppt

学到很多东西的诀窍，就是一下子不要学很多。 ------ 洛 克 微卫星DNA和AFLP标记在水稻分子标记连锁图上的分布 1熊立仲　1王石平　1刘克德　1戴先凯 2M.A.SaghaiMaroof　3胡锦国　1张启发 1(华中农业大学作物遗传改良国家重点实验室　武汉　430070) 2(Department of Crop and Soil Environmental Science, Virginia Polytechnic Institute and State University, VA24061, USA) 3(Department of vegetable Crops, University of California, Davis, CA91616,USA) 评论员：李宇歌 摘要 以一个栽培稻(Oryza sativa L.ssp.indica) 和野生稻(O.rufipogon Griff)杂交的Ｆ2作图群体以及由该群体构建的RFLP标记连锁图,分析了微卫星DNA和AFLP标记的多态性、遗传行为及其在染色体上的分布。共定位了28个微卫星ＤＮＡ标记和172个AFLP标记。28个微卫星ＤＮＡ标记中有6个为华中农业大学作物遗传改良国家重点实验室根据数据库中序列而设计,其余22个来自美国Cornell大学已发表的结果。172个AFLP标记出自25对引物扩增得到的228个多态性带的片段。这些标记分布于水稻的12条染色体。将此200个PCR标记与华中农业大学作物遗传改良国家重点实验室构建的RFLP连锁图整合,得到一张含612个分子标记位点的遗传连锁图。 关键词 SSR,AFLP,RFLP,基因图谱 Abstract ①SSR(simple sequence repeat) and AFLP (amplified fragment length polymorphism) are PCR based molecular markers developed in recent years. ②In this study, the authors analyzed the polymorphisms, inheritance and distribution of SSR and AFLP markers using an F2 population from across between cultivar “Aijiao Nante” (Oryza sativa L. ssp. Indica) and an accession of the common wild rice (O. rufipogon Griff). ③A total of 200 new markers were obtained including 28 SSR and 172 AFLP markers. ④ Six of the 28 SSR markers were developed by National Key Laboratory of Crop Genetic Improvement(NKLCGI) using DNA sequences from GenBank and the other 22 were from data published previously. ⑤The 172 AFLP markers were from a total 228 polymorphic bands amplified using 25 selected primer combination. ⑥Mapping of the 200 new markers using NKLCGI’S previously developed RFLP map based on the same F2 population resolved these markers to all 12 rice chromosomes. ⑦Integration of the SSR and AFLP markers into the RFLP map resulted in a high density molecular linkage map containing 612 polymorphic loci. 标题: ◆微卫星DNA和AFLP标记在水稻分子标记连锁图上的分布 ◆ DISTRIBUTION OF SIMPLE SEQUENCE REPEAT AND AFLP MARKERS IN MOLECULAR LINKAGE MAP OF RICE 改正：