小鼠上胚层多能干细胞研究进展-中国细胞生物学学报.pdf

中国细胞生物学学报 Chinese Journal of Cell Biology 2017, 39(7): 931–938 DOI: 10.11844/cj cb.2017.07.0362 综述 小鼠上胚层多能干细胞研究进展 * * 陈杨林 刘悦石 李喜和 包斯琴 ( 内蒙古大学, 蒙古高原动物遗传资源研究中心, 呼和浩特01002 1) 摘要 小鼠上胚层干细胞(mouse epiblast stem cell, mEpiSC)是一种来自于5.5天~7.5天小鼠 胚胎上胚层(epiblast)组织的多能性干细胞。EpiSC 自我更新能力和多能性的维持主要依赖激活素 (Activin/Nodal)和成纤维细胞生长因子(fibroblast growth factor, FGF)信号, Activin和FGF信号对细 “ 胞多能网络进行调控, EpiSC培养体系和分离方法目前仍处于不断优化的过程。相比于处于 幼 ” “ 稚(naïve) 状态的小鼠胚胎干细胞(mouse embryonic stem cell, mESC), mEpiSC被认为处于 待发 ” (primed) 状态。虽然mEpiSC被注射入囊胚后, 细胞嵌合效率较低, 但是当其被移植入着床后胚胎 时, 却比mESC更容易与受体胚胎发生嵌合。因此, 来源于小鼠着床后胚胎的EpiSC与来自于着床 前胚胎的ESC相比, 在多能性维持、发育潜能和诱导方法等方面存在本质区别。该文对小鼠EpiSC 和ESC进行比较, 并综述目前小鼠EpiSC的研究进展。 关键词 EpiSC; mESC; 多能性; 发育潜能 Advances in Mouse Epiblast Stem Cell Chen Yanglin, Liu Yueshi, Li Xihe*, Bao Siqin* (Research Center for Animal Genetic Resources of Mongolia Plateau College of Science, Inner Mongolia University, Hohhot 010021, China) Abstract Mouse epiblast stem cell (mEpiSC) is derived from the epiblast tissue of mouse 5.5 days- 7.5 days embryos. Self-renewal and pluripotency of EpiSC is maintained by Activin/Nodal and FGF (fibroblast growth factor). Activin and FGF regulate pluripotency network. Culture system of EpiSC and separation methods still being explored. Compared with “naïve” state of mouse embryonic stem cell (mESC), mEpiSC is thought to be in a state of “prim