TIMP1蛋白表达影响.docVIP

PAGE PAGE 1 TIMP-1蛋白表达影响 　　摘要：目的：观察补肺汤对肺纤维化大鼠肺组织中基质金属蛋白-9（MMP-9）和组织基质金属蛋白酶抑制剂-1（TIMP-1）蛋白表达及肺组织病理变化的影响。方法：雄性SD大鼠，随机分成4组：空白组、模型组、强的松组、补肺汤组。除空白组外，其余3组均采用博莱霉素（BLM）制作肺纤维化大鼠模型，每日灌胃给药，造模后7、14、28天取材。通过免疫组织化学染色方法，检测肺组织MMP-9、TIMP-1蛋白表达，采用光学显微镜观察肺组织病理变化。结果：与模型组比较，补肺汤组、强的松组各时间点肺泡炎和肺纤维化程度均较轻；与模型组比较，各时间点强的松组、补肺汤组肺组织MMP-9蛋白表达明显减低；与模型组比较，各时间点的强的松组、补肺汤组肺组织TIMP-1蛋白表达明显降低。结论：补肺汤能够抑制早期肺泡炎的发生，降低肺组织MMP-9与TIMP-1蛋白表达，纠正MMP-9/TIMP-1失衡，改善细胞外基质的异常代谢，从而抑制或延缓博莱霉素致大鼠肺纤维化的发生和发展。 　　关键词：肺纤维化;MMP-9;TIMP-1;补肺汤 　　中图分类号：R285.5文献标识码：A文章编号：1673-7717(2011)12-2673-07 　　StudyonEffectsofBufeiDecoctiononMMP-9andTIMP-1in 　　LungTissueofPulmonaryFibrosisRats 　　ZHANGHong,XIAYongliang,YANGJunchao,SONGKang 　　(ThefirstclinicalmedicalcollegeofZhejiangChineseMedicalUniversity,Hangzhou310006,Zhejiang,China) 　　Abstract:Objective:Toobservedynamicallytheeffectsofpolygonumcuspidateontheexpressionoftumornecrosisfactor-α(TNF-α)andplatelet-derivedgrowthfactor(PDGF)inthebronchoalveolarlavagefluid(BALF)ofpulmonaryfibrosisratsandthepathologicalchangesoflungtissue.Methods:96SDmaleratsweredividedintofourgroupsrandomlyincludingcontrolgroup,modelgroup,prednisonegroupandBufeiDecoctiongroup(n=24).Exceptthenormalcontrolgroup,other3groupswerealladoptedbleomycin(BLM)tosetupratmodelwithpulmonaryfibrosisbyintragastricadministrationeveryday,andthematerialsweredraw7,14,28daysaftermakingmodels.ThecontentsofMMP-9andTIMP-1oflungtissueweredetectedwithwithimmunohistochemicalstainingmethodandthepathologicalchangesoflungtissuewereobservedwithopticalmicroscope.Results:Comparedwithmodelgroup,thedegreeofalveolitisandpulmonaryfibrosisinBufeiDecoctionandprednisonegroupwasmildcomparedwithmodelgroup,theexpressionsofMMP-9andTIMP-1weredecreasedsignificantlyinBufeiDecoctionandprednisonegroup.Conclusion:BufeiDecoctioncandecreasethedegreeofalveolitisandpulmonaryfibrosisdecreasedsignificantlyinthedexamethasonegroupandBufeiDecoctioncoulddecreasetheexpressionsofMMP-9andTIMP-1inlungtissueandreestablishthebalancebetweenMMP-9andTIMP-1,thenimprovetheabnormalme