圆二色谱原理与应用.pptVIP

从CD谱分析准确性 对全?、 ? /?和变性蛋白质的准确度90-100% 对? + ?的准确度为85% 对全?的准确度为75% Applications of CD in structural biology Determination of secondary structure of proteins that cannot be crystallised Investigation of the effect of e.g. drug binding on protein secondary structure Dynamic processes, e.g. protein folding Studies of the effects of environment on protein structure Secondary structure and super-secondary structure of membrane proteins Study of ligand-induced conformational changes Carbohydrate conformation Investigations of protein-protein and protein-nucleic acid interactions Fold recognit