异丙酚可以防止神经元缺血再灌注引起的线粒体DNA缺失和损伤的主要脑损伤.pdfVIP

b r a i n r e s e a r c h 1 5 9 4 ( 2 0 1 5 ) 1 0 8 – 1 1 4 Available online at /locate/brainres Research Report Propofol prevents neuronal mtDNA deletion and cerebral damage due to ischemia/reperfusion injury in rats n Zi-yong Yue, Hong Dong, Ya-fang Wang, Ying Liu, Chun-yu Song , Wan-chao Yang, Hua Qian, Shu-jun Lu, Fei-fei Chang Department of Anesthesiology, China and Heilongjiang Key Laboratory for Anesthesia and Critical Care, The Second Affi liated Hospital of Harbin Medical University, Harbin 150081, China a r t i c l e i n f o a b s t r a c t Article history: Propofol is a commonly used intravenous anesthetic that has been demonstrated to be Accepted 11 October 2014 neuroprotective against cerebral ischemia–reperfusion (I/R) injury. It remains unclear Available online 11 November 2014 whether this protective effect has any relationship with the prevention of neuronal mitochondrial deoxyribonucleic acid (mtDNA) deletion. In this study, 81 Wistar rats were Keywords: randomly divided into three groups (n ¼ 27 each): sham (S group), ischemia/reperfusion (I/R Mitochondrial DNA group), or propofol (P group). Cerebral ischemia was induced by clamping the bilateral Polymerase chain reaction common carotid arteries for 10 min. A polymerase chain reaction (PCR) was conducted to Whole cerebral ischemia/