表面增强荧光研究进展_吕凤婷.pdfVIP

19 2 3 化　学　进　展 Vol.19 No .2 3 2007 3 PROGRESS IN CHEMISTRY 　Ma ., 2007 表面增强荧光研究进展＊ 1 2 1 ＊＊ 吕凤婷 　郑海荣 　房　喻 (1.陕西师范大学化学与材料科学学院大分子科学陕西省重点实验室　西安710062; 2.陕西师范大学物理学与 息技术学院　西安710062) 　　本文系统地介绍了金属表面增强荧光现象、产生机理及其应用。粗糙金属和超薄光滑金属表 面均可表现出荧光增强效应, 但这两种表面增强荧光产生的机理不同, 所发出的荧光性质也不同。粗糙金属 表面增强荧光的主要原因被认为是加快了处于基质表面附近的荧光物种的辐射衰减过程, 而超薄光滑金属 表面增强荧光则是激发态荧光物种与金属表面的等离子体耦合共振的结果。 与粗糙金属表面增强荧光不 同, 超薄光滑金属表面增强荧光具有突出的方向性。 金属表面增强荧光已经开始在DNA 无损检测、荧光共 振能量转移免疫分析等领域获得重要应用。 　金属增强荧光　辐射衰减工程　辐射等离子体模型　表面等离子体耦合发射 :644.1　:A 　:1005-281X (2007 )02 3-0256-11 Studies of Surface-Enhanced Fluorescence 1 2 1 ＊＊ LǜF engting 　Zheng Hairong 　Fang Yu (1.Key Labo ato y of Mac omolecula Science of Shaanxi P ovince, School of Chemist y and Mate ials Science, Shaanxi No mal Unive sity, Xian 710062, China;2.School of Physics and Info mation Technology, Shaanxi No mal Unive sity, Xian 710062, China) Abstract 　The phenomenon, mechanisms and applications of metallic su face-enhanced fluo escence a e eviewed. The phenomenon of enhanced fluo escence emission can be obse ved on both a non-continuous metallic su face and a continuous metallic su face .But the natu e of the enhancement is diffe ent.The p esence of a non-continuous metallic su face can have d amatic effects on the adiative decay ate of a nea by fluo opho e .Above a continuous metallic su face, howeve , excited fluo opho es can couple with su face plasmons, and esult in enhanced fluo escence .Compa ed with the emission nea a non-continuous metallic su face, the emission nea a continuous metallic su face is highly di ectional.Fu the mo e, su face-enhanced