野油菜黄单胞菌hpa2基因的突变和功能分析.pdfVIP

27 3 2008 9 Vol27, No3 Journal of Guangxi Agricand BiolScience Sept, 2008 : 1008-3 6 (2008) 03-0206-06 hp a 2 1, 2 1 3 1 1 姜伯乐 , 蒋国凤, 黄佩芳 , 杭小红 , 荆龙 , 1 1, 2 1, 2 杨艳 , 何勇强 , 唐纪良 ( 1 , 530005; 2 , 530005; 3 , 530005) : pK18mob X cc 800 hp a2 , PK3001 , hp a2 PK3001 , g us RT- PCR hp a2 , , hp a2 , hrp G hrp X : ; hp a2; : Q3 : A Mutagenesis and functional characterization of hp a2 gene in X anthomonas camp estr is pvcamp estris 1, 2 1 3 1 1 JIANG Bo-le , JIANG Guo-feng , HU ANG Pe-i fang , HANG Xiao-hong , JING Long , 1 1, 2 1, 2 YAN G Yan , HE Yong-qiang , T ANG J-i liang ( 1 College of Life Science and T echnology, Guangxi University , Nanning 530005, China; 2 Guangxi Key Laboratory of Subtropical Bioresources Conservat ion and Utilization, Nanning 530005, China; 3 College of Agriculture, Guangxi University, Nanning 530005, China) Abstract: Mutant PK3001 w as generated by the hp a2 gene of X ant homonas c amp estr is pv camp estr is ( X cc) mutated w ith suicide plasmid pK 18mob The pathogenicity and hypersensitive reaction ( HR) of mutant PK3001 w ere determined T he hp a2 mutant PK3001 didnt affect the virulence significantly but lost HR completely T he expression and regulation of the hp a2 gene w ere analyzed through g us report gene and RT-PCR The results show ed that the hp