《不同盐浸种向日葵萌发的影响》-毕业论文（设计）.docVIP

PAGE 2 2011届农业资源与环境本科生毕业论文 不同盐溶液浸种对向日葵酶活性及萌发的影响 PAGE 3 PAGE 1 不同盐溶液浸种对向日葵萌发的影响 摘要 分别用不同盐溶液（NaCl、NaHCO3、混合盐）对向日葵种子进行浸种处理，将浸种后的种子经低温回干，再用不同浓度的混合盐溶液（NaCl：NaHCO3=1：1）进行胁迫处理。通过向日葵种子内的淀粉酶活性的测定以及发芽率、发芽势和苗长等指标的测试，分析不同盐溶液浸种对向日葵种子萌发的影响。结果表明：经过不同盐溶液浸种处理后，淀粉酶的活性都有所增强，促进了淀粉的水解，给萌发提供充分的能量，缩短了向日葵种子的发芽时间，促进的幼苗的生长，提高了种子的活力、发芽率。未经过盐浸种处理的种子即使是在蒸馏水中培养，其酶活性、发芽率、发芽势以及平均苗长等指标均不如经过盐溶液预处理种子的。种子经过不同盐溶液浸种、回干后，在蒸馏水中萌发的各种指标最好，而后随着处理的混合盐浓度的增大，向日葵酶活性、发芽率、发芽势和总苗长逐步降低。各种盐溶液浸种的对向日葵种子酶活性和萌发的影响依次是：混合盐 NaHCO3 NaCl。 关键词 向日葵， 淀粉酶， 酶活性 ，发芽率 ，发芽势 DIFFERENT SALT SOLUTION OF SUNFLOWER SEED GERMINATION AND THEINFLUENCE OF ENZYME ACTIVITY Zeng Mingyuan LongDong University Af College Qingyang In Gansu Province Abstract In this paper, we use different salt solution (NaCl, NaHCO3, of sunflower seeds sulphonating agent.) seed handling; Will the seeds of seed by low temperature back after dry, garnish with different concentrations of NaCl: sulphonating agent.the solution (NaHCO3:NaCl = 1:1) as part of a campaign of intimidation processing. Through the sunflower seeds in the determination of amylase activity and germination rate, vigor index and germination index index test, analyze the different salt solution of sunflower seed germination triggered effects. The result shows: after different salt solution seed handling, increased amylase activity, promoted the starch hydrolysis, to provide sufficient energy and germination of seeds germinate shortened sunflower, promote the time the seedlings growth, improved seed vigor, germination rate. Without salt seed handling seeds even in distilled water training, its enzyme activity and germination rate, sprout potential and average seedlings as long indexes by the salt solution pretreatment seeds. Seeds through different salt solution soaked in dry, and return the germination of distilled water, best, then with various indexes with the increase of concentration, sunflower sulphonating agent.the germi