核酸特性、结构和功能英文版.pptVIP

19 Are Nucleosomes “Positioned?” 1. Sequence at Unique Position 2. Micrococcal Nuclease Digest 3. Restriction Digest 4. Southern Blot Discrete Band Positioning Smear Random Orientation 17 Implications of Translational Positioning 18 N-ARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTE- H3 N-SGRGKGGKGLGKGGAKRHRKVLRDNIQ - H4 N-SGRGKQGGKTRAKSKTRSSRAGL- H2A N-SDPAKSAPAAKKGSKKAVTKTQKKDGKKRRKSRKES- H2B H3 -ERA-C H4 -RTLYGFGG-C H2A -ELNKLLGGVTIAQGGVLPNIQSVLLPKKTESSKSTKSK-C H2B -PGELAKHAVSEGTKAVTKYTSAK-C Core Core Histones Have Variable Length Tails 20 H4 H2B H2A