二倍体和四倍体结球甘蓝减数分裂观察.pdf

31 2 Vol.31 No.2 20 0 8 3 JOURNAL OF AGRICU LT URAL UNIVERSITY OF HEBEI 　 　 M ar .2 0 0 8 :1000-1573(2008)02-0021-06 ① 轩淑欣, 　张成合, 　申书兴, 　陈雪平, 　李晓峰 ( , 071001) :。 :, , 、、;ⅠⅡ ;Ⅰ、;Ⅱ ;;。 　　:;;;;; :S 635.1 :A Meiotic observation of pollen mother cells in diploid and autotetraploid cabbage(Brassica oleracea var.cap itata ) XUAN Shu-x in, ZHANG Cheng-he, SHEN Shu-xing, CHEN Xue-ping, LI Xiao-feng (College of Horticulture, Agricultural University of Hebei, Baoding 071001, C ina) Abstract:Meiosis of pollen mot er cells (PMCs)in diploid and autotetraploid cabbage w ere ob- served.T e results s ow ed t at t e be avior of meiosis in autotetraploid w as muc more complicat- ed t an in diploid.At diakinesis, t e omologous c romosomes of t e autotetraploid cabbage could form quadrivalents, trivalents, bivalents and univalents;at metap ase I and II, t e univalents often distributed at t e outside equator plate;at anap ase I, t ere were lagging c romosomes, early sepa- rating c romatids, w ic result in unbalanced separation;in anap ase Ⅱ, t e number of c romo- somes at eac pole s owed great variations.Regularity and viability of pollen grains in autotetraploid were significantly lower t an t ose of diploid.Cytological reason for lower fertility of tetraploid w as dicussed. Key words:cabbage;diploid;autotetraploid;PMCs;meiosis;pollen viability 　　、 [2] 。 , (B rassica oler acea var.cap i tata ) , 、 , 。 。 50 ～60 , [3]