脑囊虫患者血清及脑脊液中细胞因子与血_脑脊液屏障相关性研究.docVIP

?297? 脑囊虫患者血清及脑脊液中细胞因子与 血2脑脊液屏障相关性研究 闫晓波,俞春江,王维治 (哈尔滨医科大学附属第二医院神经科,黑龙江哈尔滨150086) 摘要:目的　研究脑囊虫患者是否存在血2脑脊液屏障(,以期对临床诊治有所帮助。方法　用单向免疫扩散法检测41;ELISA法检测患者血清和脑 )及肿瘤坏死因子(F功能与IFN2Χ脊液(CSF)中Χ2干扰素(IFN2Χ、TNF2Α产生的关系。结 果　(1)脑囊虫患者中有8219%。(CSF中IFN2Χ水平较对照组明显降低(均P0101),TNF2Α(P0105,P0101)。(3)BBB损害组CSF中IFN2Χ水平较BBB(P,IFN2Χ无变化。(4)BBB损害组CSF中TNF2Α水平较BBB正常组无明显增高(P,而血清中TNF2Α水平较BBB正常组明显增高(P0105)。结论　脑囊虫患者多出现BBB,同时存在体液免疫增高和细胞免疫减低。 关键词:脑囊虫病;免疫;血2脑脊液屏障;细胞因子 中图分类号:R53213　文献标识码:A　文章编号:100622963(2004)0520297203 StudyoftheCorrelationbetweenCytokinsandBBBinSerumand CSFofPatientswithCerebralCysticercosis YANXiao2bo,YuChun2jiang,WANGWei2zhi (DepartmentofNeurology,TheSecondAffiliatedHospital,HarbinMedicalUniversity,HeilongjiangHarbin150086,China)ABSTRACT:Objective　Toevaluatewhethertherearesomedisordersinblood2brainbarrior(BBB)functionarecorrelatedbetweencytokinsandBBB,whichmayaidthediagnosisandtreatmentofpatientswithcerebral .Methods　BBBfunctionweredetectedbySIRDin41patients(F21,M20)withcerebralcysticercosis cysticercosis;ThelevelofIFN2ΧandTNF2ΑweretestedbyELISA.Results　(1)TherewasabnormalBBBincerebralcysticercosisgroup(82.9%).(2)TheIFN2ΧlevelofserumandCSFincerebralcysticercosisgroupdecreaseddistinctivelyascomparedwithcontrolgroup(P0101).TheTNF2ΑlevelincreaseddistinctivelyinbothserumandCSF(P0105,P0101).(3)TheIFN2ΧlevelofCSFingroupofBBBdamagedecreased .(4)ThedistinctivelyincomparisonwithBBBnormalgroup(P0105),whilethatofserumwerenormal TNF2Αlevelincreaseddistinctivelyinserum(P0105)butnotinCSF(P0105).Conclusions　ThestudysuggeststhatthereisadysfunctionofBBBandcorrelatedwithcytokinsproductioninpatientswithcerebralcysticercosisandalsoenhancementofhumoraldisimmunityandpoornessofcellulardisimmunity. Keywords:cerebralcysticercosis;immunity;blood2brainbarrior;cytokins 收稿日期:2002208223;修订日期:2002212222; 作者简介:闫晓波 (19602),女,河北省人,教授,主要从事神经免疫性疾病研究。通讯地址:哈尔滨医科大学附属第二医院神经科,哈尔滨 150086。联系电话:(0451 ?298?　　目前已有诸多研究证明脑囊虫病存在免疫异常,但对其血清及脑脊液(CSF)中Χ2干扰素(IFN2)和肿瘤坏死因子(TNF2Α)水平同时检测并观察Χ 其与血2脑脊液屏障(BBB)的关系目前尚未见报道。此研究对41例脑囊虫患者血清及CSF