氨基酸多肽和蛋白质.pptVIP

* * * * * * * * * * * * * * * * * * * * * * * Copyright ? 2006 Pearson Prentice Hall, Inc. * * * * * * * * * * * * * * * * * * * Composition of Human Bradykinin Use of an amino acid analyzer to determine the composition of human bradykinin（舒缓激肽）. Sequencing from the N Terminus: The Edman Degradation Edman degradation: The reaction with phenyl isothiocyanate followed by hydrolysis removes the N terminus amino acid. The phenylthiohydantoin derivative is identified by chromatography. Use for peptides with 30 amino acids. Edman Degradation Last Step of the Edman Degradation In the final step (