气相色谱_质谱_GC_MS_联用技术对麝香的多成分含量分析.docVIP

[分　　析] 气相色谱/质谱(G C/MS 联用技术对麝香的多成分含量分析 张皓冰1, 　何　昱2, 　贾国惠2, 　洪筱坤2, 　王智华2, 　叶愈青3 (1. 中国疾病预防控制中心寄生虫预防控制所, 上海200025;2. 上海中医药大学中药学院, 上海200032;3. 雷允上药业有限公司, 上海200002 关键词:气相色谱/质谱联用技术; 麝香 摘要:目的:旨在探索气相色谱/GC/, 法。结果:采用GC/MS , 量, 。:/质谱联用技术是象麝香那样复, 。 中图分类号:A　　　　　文章编号:100121528(2002 1120868204 Q uantitative analysis for multi 2Components of musk by gas chromatography/mass spectrometry ZHAN G Hao 2bing , 　HE Yu , 　JA Guo 2hui , 　HON G Xiao 2kun , 　WAN G Zhi 2hua , 　YE Yu 2qing (1Instit ute f or Parasitic Diease Cont ral and Prevention , CC DC , S hanghai 200025, Chi na ; 2S hanghai U niversity of TCM , S hanghai 200032, Chi na ; 3Lei Y un S hang Pharmaceutical Co. , L t d. S hanghai 200002, Chi na K ey w ords :G as chromatography/mass spectrometry (GC/MS ; musk ; quantitative analysis Abstract :Objective :This study was designed to investigate the application of gas chromatography/mass spec 2trometry for analyzing the quality of musk. Methods :Standard curves with internal standard and G as chromato 2graphy/mass spectrometry (GC/MS were utilized. R esults :Muscone , androsterone and cholesterol in both natural and man 2made musk were analyzed quantitatively simultaneously by GC/MS , which was highly selective to the components to be determined by selecting the characteristic fragments. The study of analytical methodolo 2gy proved that the method above was practical and accurate. Conclusion :G as chromatography/mass spectrome 2try is a powerful arm to analyze the complex system such a musk , which has a promising perspective in quality control of traditional Chinese medicinal materials. 收稿日期:2002207216 作者简介:张皓冰(1962～ , 女, 博士, 副研究员, 药物室主任。电话:(021 6437700822603; Email :zhanghaobing@ 　　麝香众多组分为低级性, 小分子量、具挥发性的物质, 含量测定多采用气相色谱法, 用量少, 方法灵敏。气相色谱定量一般为标准曲线法[1]和内标法。特别是内标法, 应用尤其广泛, 较多用正十四烷、正十七烷[2]、正十八烷[3]、正十九烷[4]、萘[5]等为内标。 本研究采用GC/MS 联用法, 以睾丸酮为内标, 实现了对多个天然麝香样品中多种成分的定性定量分析。1　实验部分 1. 1　药品与仪器1. 1. 1　样品与试剂 天然麝香:1号～5号样品(原麝麝香 ; 6号、7 号样品(麝香仁 ;1号～7号样品由雷允上中药研究所提供;8号样品:由四川药品检验所提供; 9号样品:购至青海;10号样品:购自医药公司;