球囊损伤血管内皮后平滑肌细胞凋亡及相关基因表达的变化.docVIP

???? 球囊损伤血管内皮后平滑肌细胞凋亡?及相关基因表达的变化 ???? ????［摘　要］　目的：探讨球囊损伤血管内皮后平滑肌细胞(SMC)凋亡的机制。方法：采用末端脱氧核苷酸转移酶介导的三磷酸脱氧尿嘧啶缺口末端标记法(TUNEL)和免疫组织化学技术检测球囊损伤内皮后血管平滑肌细胞(VSMC)凋亡及Bax、Bcl-2蛋白的变化。结果：球囊损伤内皮后第3 d，血管中层出现凋亡的SMC； 损伤后第7 d，内膜和中层SMC凋亡率最高，凋亡的SMC主要分布在内膜层；以后逐渐降低，至损伤后第28 d，仅内膜层有少量凋亡的SMC。Irbesartan显著增加SMC凋亡(P＜0.01)。球囊损伤内皮后第3 d，血管中层Bax、 Bcl-2表达显著高于假手术组(P＜0.01)；损伤后第7 d血管中Bax表达最高，是假手术组的3倍，以后表达减少。球囊损伤内皮后Bcl-2表达逐渐增多，至第28 d表达最高。Bax/Bcl-2也在损伤后第7 d达最高，至第28 d降至基础水平以下。Irbesartan使Bax表达增高、Bcl-2表达降低、Bax/Bcl-2升高。结论：Bax、Bcl-2参与了球囊损伤内皮后VSMC凋亡的调节。　　［主题词］　肌，平滑， 血管； 内皮，血管； 大鼠　　［中分类号］　R543　　　［文献标识码］　A［文章编号］　1000-4718(2000)08-0694-04 The change of smooth muscle cells apoptosis and apoptosis related-gene expression after balloon injury to vessel WANG Geng, HE Guo-xiang, CHI Lu-xiang, ZHANG Guo-qiang(Department of Cardiology, Southwest Hospital, the Third Military Medical University, Chongqing 400038, China) 　　［Abstract］　AIM: To investigate the mechanism of vascular smooth muscle cells (VSMC) apoptosis after balloon injury. METHODS: VSMCs apoptosis, Bax protein and Bcl-2 protein was measured by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) and immunohistochemical technique after balloon injury.RESULTS: VSMCs apoptosis occurred in vascular media at 3 days after balloon injury and reached a peak in media and intima where apoptosis was mainly present at 7 days, then decreased. At 28 days after balloon injury, only a few apoptosis cells were in intima. Irbesartan significantly increased VSMCs apoptosis (P＜0.01). Expressions of Bax and Bcl-2 were significantly higher in vascular media at 3 days after balloon injury than sham(P＜0.01). At 7 days, Bax expression reached a peak which was three times as that of sham, and then decreased. After balloon injury, Bcl-2 expression was generally increased and reached its peak at 28 days. The ratio of Bax to Bcl-2 (Bax/Bcl-2) also reached its peak at 7 days and was decreased under basal level at 28 days. Irbesartan increased Bax and t