NADPH氧化酶源性活性氧介导epinodosinHL-60细胞分化及.PDFVIP

中国细胞生物学学报 Chinese Journal of Cell Biology 2016, 38(3): 273–284 DOI: 10.11844/cj cb.2016.03.0368 NADPH氧化酶源性活性氧介导epinodosin诱导 HL-60细胞分化及细胞骨架重组 * 丁 兰 陈宗儒 陈 平 李佩蔚 刘国安 (西北师范大学生命科学学院, 兰州730070) − 摘要 该文分析研究了对映 贝壳杉烷二萜epinodosin通过调节NADPH氧化酶源活性氧诱导 急性早幼粒细胞白血病细胞HL-60向成熟粒细胞分化的特征。结果显示, epinodosin在4.0~8.0 μmol/L 浓度时抑制HL-60细胞生长, 导致其S期阻滞, 诱导HL-60细胞形成肾形核和多叶核细胞, 使细胞吞 噬能力和对硝基四氮唑蓝(nitrotetrazolium blue chloride, NBT)的还原力显著增强、细胞表面抗原 CD 11b表达上调, 表明epinodosin可诱导HL-60细胞向成熟粒细胞分化; epinodosin还诱导HL-60细胞 骨架重组, 显著促进微管的成束组装以及提高波形纤维含量, 上述分化特征与临床诱导分化治疗 剂全反式维甲酸(all-trans retinoic acid, ATRA)对HL-60细胞的分化诱导特性极为相似。进一步检测 显示, 抗氧化剂NAC(N-acetyl-L-cysteine) 以及NADPH氧化酶抑制剂夹竹桃麻素(apocynin, APO)和 联苯基三价碘(diphenyleneiodonium, DPI)均可抑制epinodosin对HL-60细胞的分化诱导作用, 表明 epinodosin导致HL-60细胞活性氧浓度的升高与NADPH氧化酶活性关联, 提示epinodosin通过调节 NADPH氧化酶源性活性氧诱导HL-60向粒细胞分化。 关键词 epinodosin; HL-60细胞; 分化; 活性氧; NADPH氧化酶; 细胞骨架重组 NADPH Oxidase-derived ROS Mediated the Differentiation and Reorganization of Cytoskeletalon Induced by Epinodosin in HL-60 Cells Ding Lan*, Chen Zongru, Chen Yiping, Li Peiwei, Liu Guoan (College of Life Science, Northwest Normal University, Lanzhou 730070, China) Abstract In this paper, NADPH oxidase-derived reactive oxygen species (ROS) mediated the differentiation of HL-60 to mature granulocyte caused by epinodosin were studied. The results showed that the growth of HL-60 was inhibited by epinodosin under the concentration of 4.0~8.0 μmol/L, and it was arrested at S phase. Epinodosin induced HL-60 formed kidney-shaped nuclei and lobulated nuclei, enhanced nitrotetrazolium blue chloride (NBT)-reducing ability, cellular uptake and the expression of CD1