分光光度法测定丝肽液中氨基酸总含量-.docVIP

F LAV OUR FR AGR AN CE C OSMETICS 　Ju ne ,2011,N O 13 分光光度法测定丝肽液中氨基酸总含量 作　者 1.化学化工与生物工程学院,生态纺织教育部重点实验室,东华大学,上海　201620; 2.工业品与原材料检测技术中心,上海出入境检验检疫局,上海　200135黄威娜1,2　杨　勇2　林　苗1 ………………………………摘　要 　　采用分光光度法测定丝肽液中氨基酸总含量,确定以甘氨酸为标准品、茚三酮为显色剂的测定方法。最佳反应条件:反应p H 值为5.4,缓冲液的用量为2.0mL ,显色剂的用量为1.0mL ,沸水浴加热15min ,冷却至室温后,在最大吸收波长 573nm 处测定吸光度。该方法操作简便、结果准确,重现性良好,可用于化妆品产品的检验。 关键词 丝肽液　茚三酮　含量测定　分光光度法 Determination of Amino Acid Content in Silk Peptide Solution by Spectrophotometry H UA N G W ei na 1,2 　YA N G Yong 2　L I N M i ao 1 (1.College of Chemist ry ,Chemical Engineering and Biotechnology ,Key Laboratory of Science Tech 2 nology of Eco 2Textile of Ministry of Education ,Donghua University ,Shanghai 201620,China ;2.Inspec 2tion Center of Indust rial Product Raw Materials ,Shanghai Entry 2Exit Inspection Quarantine Bureau ,Shanghai 200135,China Abst ract :　The total amount of amino acid in silk peptide solution was analyzed by spectrometer.The measuring method was investigated ,using glycine as the standard substance and ninhydrin as the chromogenic agent.The optimum conditions were proved as follows :buffer solution volume 2mL with p H of 5.4,chromogenic agent volume 1mL ,heating time 15minutes in boiling water bath ,absorption wavelength at 573nm after the sample solutions were cooled to the room temperature.It was shown that this method was a simple ,accurate ,and repeatable measurement ,which could be adopted to supervise and examine the amino acid content of cosmetic products. Key wor ds :　silk peptide solution 　ninhydrin 　content determination 　spectrophotometry 收稿日期:2010209224 　　丝肽[1]是天然蚕丝的水解产物,其基本结构单元是氨基酸。分子中含有大量的氨基(2N H 2、羧基(2COO H 、羟基(2O H 等亲水性基团,能够像手一样抓住水分子,将水束缚住,具有优异的吸湿保湿作用。丝肽可以起到营养肌肤、延缓衰老、减少皱纹的功效,还能抑制酪氨酸酶的活性,减少黑色素形成,使皮肤白嫩。丝肽优异的保湿作用及独特的护肤特性正在被越来越多的人接受和认可,在 化妆品中的应用日益普遍。测定丝肽液中氨基酸的含量,是丝肽类化妆品质量评价中最为重要的指标。 根据氨基酸分析仪的工作原理和相关氨基酸的检测方法,以甘氨酸为标准品,茚三酮为显色剂,分光光度计为测定仪器,对紫外分光光度法测定氨基酸含量的方法条件和可行性进行了一系列的研究,得到了比较满意的结果。该方法所用试剂配制简单、无毒害、操作过程简便、稳定性好,相关性在0.999以上,回收率在95%~105%之间。 1　仪器与试剂 1.1　仪器 AB2042N