水杨酸对水分胁迫下草莓幼苗膜脂过氧化的影响.docVIP

华北农学报·2010, 25(5:127-131收稿日期:2010－08－10 基金项目:国家自然基金资助项目内蒙古大学博士后基金资助项目(207036;内蒙古自然基金资助项目(200711020314作者简介:刘艳(1971－,女,内蒙古呼和浩特人,副教授,博士,主要从事植物逆境生理与分子生物学方面的研究。 水杨酸对水分胁迫下草莓幼苗膜脂过氧化的影响 刘 艳 1,2 ,陈贵林1,李晓燕2,刘景秀 2 (1．内蒙古大学生命科学学院,内蒙古呼和浩特 010021;2．内蒙古农业大学农学院,内蒙古呼和浩特 010019 摘要:研究水杨酸在诱导植物抗旱性形成中的作用。以草莓幼苗为试材,采用不同浓度外施水杨酸预处理,测定水分胁迫下各处理草莓叶片膜脂过氧化相关指标,保护酶类活性和光合特性。水分胁迫下,未施水杨酸的草莓叶片活性氧出现一高一低2个峰值,第1个峰值之后保护酶类活性显著提高,膜脂过氧化指标维持较低水平;随着胁迫时间延长,活性氧再次小幅积累,膜脂过氧化程度随之加剧。适宜浓度水杨酸预处理可以促进水分胁迫下活性氧的早期积累,并有效激活保护酶活性,减轻膜脂过氧化损伤,叶片维持高光合活性;但高浓度水杨酸预处理会加剧膜脂过氧化损伤。水杨酸通过促进活性氧信号泵发, 活化保护酶活性,减轻膜脂过氧化损伤来提高植物对水分胁迫的抗性。关键词:草莓;水杨酸;水分胁迫;膜脂过氧化中图分类号:S668．4 文献标识码:A 文章编号:1000－7091(201005－0127－05 The Influence of the Salicylic Acid for Membrane Lipid Peroxidation under Water Stress on Strawberry Young Plants LIU Yan 1,2 ,CHEN Gui-lin 1,LI Xiao-yan 2,LIU Jing-xiu 2 (1．College of Life Science ,Inner Mongolia University ,Huhhot 010021,China ; 2．College of Agronomy ,Inner Mongolia Agricultural University ,Huhhot 010019,China Abstract :Effects of salicylic acid in plant drought resistance were investigated．We take strawberry young plants as the materials and use the pretreatment of different concentrations of salicylic acid , each processing strawberry leaves were determined plasmalemma peroxide index , protective enzymes activity and photosynthetic characteristics．under water stress ,the reactive oxidative species (ROS of strawberry leaves appears two different peaks．The antioxidant en-zymes obvious increase after the first peak ,and the membrane lipid peroxidation maintain lower level ;with the stress time prolong ,ROS accumulates again ,and membrane lipid peroxidation increase immediately with it．Suitable concen-tration salicylic acid treatments promote the ROS earlier period accumulation and effectively stimulate antioxidant en-zymes activity ,and alleviate membrane lipid peroxidation damage ,also leaves maintain high photosynthesis activity ;but high concentration salicylic acid treatments promote membrane lipid peroxidat