用MacELISA法检测病毒性心肌炎患者血清中柯萨奇B组病毒IgM抗体.docVIP

???? 用Mac?ELISA法检测病毒性心肌炎患者?血清中柯萨奇B组病毒IgM抗体 ???? ????【摘要】　目的　了解北京地区病毒性心肌炎的病毒感染状况。方法　用IgM抗体捕捉ELISA(Mac ELISA)，检测193例临床诊断为病毒性心肌炎患者血清中的Cox B 1～6型病毒特异性IgM抗体。结果　Cox B病毒IgM抗体阳性检出率为69.9%(135/193)，其中阳性检出率最高为B3型，占40%(54例)；其余依次为B2型，占29.6%(40例)；B4型占18.5%(25例)；B1型占7.4%(10例)；B5型占3.0%(4例)；B6型占1.5%(2例)。而181例同年龄组非心肌炎患者IgM抗体阳性检出率为20.4%(37/193)。病毒性心肌炎患者血清中的Cox B IgM抗体阳性检出率明显高于非心肌炎患者，两者差异有显著性，U＝9.56，P＜0.01。结论　Cox B感染是病毒性心肌炎的主要病因。　　【主题词】　柯萨奇病毒B组；　心肌炎；　酶联免疫吸附测定；　IgM Detection of Coxsackie B virus IgM antibody in myocarditis patients by Mac ELISA ZHU Hui, ZHANG Libi（Institute of Virology, Chinese Academy of Preventive Medicine,Beijing 100052, China） 　　【Abstract】　Objective　To detect Coxsackie B virus type 1-6 IgM antibodies from 193 clinically diagnosed myocarditis cases. Methods　The capture IgM ELISA (Mac ELISA) test wass used. Results　The total positive rate of Coxsackie virus B 1-6 IgM antibody in myocarditis patients was 69.6% (135 cases), among which Cox B3 type was as high as 40% (54 cases) of the total positive patients. The others were 29.6% (40 cases),18.5% (25 cases),7.4%(10 cases),3.0% (4 cases), 1.5%(2 cases) of Cox B2,Cox B4,Cox B1,Cox B5 and Cox B6 respectively. Meanwhile only 20.4% (37 cases) Cox B IgM positive cases could be detected in 181 non-myocarditis patients of same ages. The results showed that the positive rate of Cox B virus IgM antibody in clinically diagnosed myocarditis was significantly higher than that in control group (P＜0.01). Conclusion　The Cox B viruses may be the main agents causing viral myocarditis in Beijing cases. Mac ELISA is an easy， rapid，specific and sensitive method to detect Cox B virus IgM antibody for early and rapid diagnosis of myocarditis.　　【Key words】　Coxsackie B viruses;　Myocarditis;　Enzyme-linked immunosorbent assay;　IgM 　　柯萨奇B组病毒(Cox B)隶属于小RNA病毒科，其感染可引起人类病毒性心肌炎、无菌性脑膜炎、新生儿全身性感染等多种疾病〔1〕。据近年来报道急性病毒性心肌炎和心包炎多数情况下是由柯萨奇B组病毒引起的。这类感染在免疫功能正常的患者最初临床症状易被忽视，一部分病例可以自愈，但也可能发展成严重的症状〔2〕。临床上主要靠检测特异性IgM抗体分析其可能病因。本文即报道了Cox B 1～6型IgM抗体的检测结果。 1　材料和方法