水分胁迫对不同抗旱性小麦品种芽根生长过程中IAA, ABA 含量的影响.docVIP

第26卷第6期作　物　学　报V o l.26,N o.6 2000年11月A CTA A GRONOM I CA S I N I CA N ov.,2000 水分胁迫对不同抗旱性小麦品种芽根生长过程中I AA、ABA 含量的影响X 王　玮1　李德全1　杨兴洪1　邹　琦1　周　燮2　杨　军2 (1山东农业大学植物科学系,山东泰安271018;2南京农业大学农学系,江苏南京210095 提　要　用30%的PEG26000模拟干旱条件,对抗旱性强的北农2号和抗旱性弱的921842萌发期的小麦幼苗根系进行水分胁迫处理,分别在处理后0、3、8、20、32、45h测定了I AA、ABA含量及芽、主胚根的长度、含水量。结果发现:水分胁迫引起两个品种根、芽中ABA含量大量增加,抗旱性强的北农2号ABA反应较抗旱性弱的921842快。在胁迫3h,北农2号的根、芽中ABA就有开始增加;而921842在胁迫8h才开始增加。胁迫条件下,921842芽中ABA的含量较北农2号高。较对照增加幅度大。水分胁迫下两个品种芽中I AA含量均有降低的趋势,921842降低相对较多;但胁迫引起了北农2号根中I AA含量在3h和8h大幅度升高,随后下降。921842在胁迫3h I AA含量稍有升高,随后降低到低于对照的水平。无论是胁迫条件下,还是正常水分条件下,根中I AA含量的峰值均在芽之前。水分胁迫对芽生长的抑制与ABA含量的升高和I AA含量的降低有关,但根系生长与两种内源激素的关系则较复杂。 关键词　I AA;ABA;水分胁迫;小麦 Effects of W a ter Stress on L evel Changes of I AA and ABA i n Root and Shoot of D ifferen t D rought Resistance W hea t W AN G W ei1　L I D e2Q uan1　YAN G X ing2Hong1　Z OU Q i1　ZHOU X ie2　YAN G Jun2 (1D ep art m ent of P lant S ciences,S hand ong A g ricu ltu ral U niversity,T aian271018;2D ep art m ent of A g rono my,N anj ing A g ricu ltu ral U niversity,N anj ing210095 Abstract　　D esiccati on2stress conditi on s w ere app lied to the roo ts of w in ter w heat u sing 30%(w vpo lyethylene glyco l(PEG.T he levels of indo le232acetic acid(I AAand ab scisic acid(ABAin roo ts and shoo ts of w heat w ith strong drough t2resistance and w eak drough t resistance w heat w ere determ ined by EL ISA after w ater stress.T he enhancem en t in ABA in bo th roo ts and shoo ts du ring w ater stress w as ob served,T he largest increased con ten ts of ABA w ere found in roo ts at3h and in shoo ts at20h after treatm en t in B einong N o.2(a cu ltivar resistan t to drough t,resp ectively.A nd that w ere found at8h after w ater stress treatm en t in bo th roo t and shoo t in921842(a cu ltivar no resistan t to drough t.B u t the level of I AA in roo t and shoo t w ere very differen t,the level of I AA in roo t increased strongly at 8h after treatm en t in B einong N o.2,then decreased.How ever,there is on ly a little increase at3h in92