血管生成抗体芯片研究麦冬多糖对内皮细胞瘦素表达的影响.docVIP

血管生成抗体芯片研究麦冬多糖对内皮细胞瘦素表达的影响 王硕1， 冯怡1 *， 徐德生2， 章漳1， 丁侃3 （1 上海中医药大学中药学院 上海201203；2 上海中医药大学附属曙光医院上海200021；3 中国科学院上海药物研究所 上海201203） 摘要 目的：应用血管生成抗体芯片技术探讨麦冬多糖MDG-1对体外培养的人微血管内皮细胞 (HMEC-1) 瘦素表达的影响。方法：使用血管生成抗体芯片技术检测不同浓度MDG-1处理后人微血管内皮细胞中多种生长因子的变化情况，确定与其治疗冠心病和糖尿病作用相关的生长因子；然后利用RT-PCR方法检测MDG-1对瘦素mRNA表达的影响。结果：抗体芯片结果显示MDG-1对多种生长因子都有调节作用，其中0.2、1、10 mmol/L MDG-1对瘦素蛋白表达均有明显的抑制作用，RT-PCR结果也表明MDG-1可以抑制瘦素mRNA表达。结论： MDG-1可以显著抑制人微血管内皮细胞瘦素mRNA和蛋白的表达，MDG-1治疗冠心病和糖尿病作用可能与调节瘦素表达有关。 关键词 麦冬多糖；内皮细胞；瘦素；血管生成抗体芯片 中图分类号：R285.5，R587.1文献标识码：A Effect of Polysaccharide from Ophiopogon japonicus on the expression of leptin in endothelial cells by using Angiogenesis Antibody Arrays WANG Shuo1, FENG Y11*, XU De-sheng2, ZHANG Zhang1, DING Kan3 (1Department of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China；2Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200021, China；3Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China) 资助项目：上海市教委重点学科（No. J50302） 作者简介：王硕，男，博士研究生 * 通讯作者：冯怡，女，教授，博士生导师 Tel：021 E-mail: fyi@ ABSTRACT OBJECTIVE: To investigate the effect of a polysaccharide from O. japonicus (MDG-1) on the expression of leptin protein and mRNA in human microvascular endothelial cells (HMEC-1) by using Angiogenesis Antibody Arrays. METHODS: To find out the growth factors relative with the treatment of MDG-1 on coronary heart disease and diabetes, the expressions of 19 cytokines were evaluated using Angiogenesis Antibody Arrays with 0.2、1、10 mmol/L of MDG-1 in HMEC-1 cells. Then, the expression of leptin, the growth factor determined by Angiogenesis Antibody Arrays, mRNA was detected by RT-PCR．RESULTS: After treated with MDG-1 the expression of leptin protein and mRNA in HMEC-1cells was significantly suppressed in comparison with control group (P 0.01)．CONCLUSION: MDG-1 could significantly suppress the expression of leptin protein and mRNA in HMEC-1cells and leptin might play a role in the treat